PDF(Pubmed)
Abstract：
BACKGROUND: Arboviruses are a group of viruses transmitted to vertebrate hosts by certain blood-feeding arthropods. Among urban vectors of arboviruses, mosquitoes of the genus Aedes are the most common. However, other mosquitoes may be susceptible to infection and involved in the transmission, such as Mansonia spp. Therefore, this study aimed to investigate whether Mansonia humeralis can be infected with the Mayaro virus (MAYV).
METHODS: These insects were collected from 2018 to 2020 in chicken coops of rural communities in Jaci Paraná in Porto Velho, Rondônia, Brazil, while performing blood-feeding on roosters. The mosquitoes were randomly grouped in pools from which the head and thorax were macerated and checked for the presence of MAYV by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The positive pools were used to infect the C6/36 cell line, and on different days post-infection, the supernatant of the infected cells was subjected to viral detection by RT-qPCR.
RESULTS: A total of 183 pools of female mosquitoes were tested, of which 18% were positive for MAYV; some samples from insect pools inoculated into C6/36 cells showed in vitro multiplication capacity between 3 and 7 days post-infection.
CONCLUSIONS: This is the first report of Ma. humeralis mosquitoes that are naturally infected by MAYV, indicating that these vectors may be potential transmitting agents of this arbovirus.
METHODS: These insects were collected from 2018 to 2020 in chicken coops of rural communities in Jaci Paraná in Porto Velho, Rondônia, Brazil, while performing blood-feeding on roosters. The mosquitoes were randomly grouped in pools from which the head and thorax were macerated and checked for the presence of MAYV by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The positive pools were used to infect the C6/36 cell line, and on different days post-infection, the supernatant of the infected cells was subjected to viral detection by RT-qPCR.
RESULTS: A total of 183 pools of female mosquitoes were tested, of which 18% were positive for MAYV; some samples from insect pools inoculated into C6/36 cells showed in vitro multiplication capacity between 3 and 7 days post-infection.
CONCLUSIONS: This is the first report of Ma. humeralis mosquitoes that are naturally infected by MAYV, indicating that these vectors may be potential transmitting agents of this arbovirus.
摘要：
背景：虫媒病毒是一组通过某些吸血节肢动物传播给脊椎动物宿主的病毒。在虫媒病毒的城市媒介中，伊蚊属的蚊子是最常见的。然而,其他蚊子可能容易感染并参与传播，比如Mansoniaspp.因此,本研究旨在调查肱骨曼索氏菌是否可以感染Mayaro病毒（MAYV）。
方法：这些昆虫是从2018年至2020年在Velho港JaciParaná农村社区的鸡舍中收集的，朗多尼亚,巴西,同时对公鸡进行血液喂养。将蚊子随机分组在池中,从池中浸渍头部和胸部,并通过定量逆转录聚合酶链反应(RT-qPCR)检查MAYV的存在。阳性池用于感染C6/36细胞系，在感染后的不同日子，通过RT-qPCR对感染细胞的上清液进行病毒检测。
结果：总共测试了183池雌性蚊子，其中18％对MAYV呈阳性；来自接种到C6/36细胞中的昆虫池的一些样品在感染后3至7天之间显示出体外增殖能力。
结论：这是Ma的第一份报告。被MAYV自然感染的肱骨蚊子，表明这些载体可能是这种虫媒病毒的潜在传播因子。
方法：这些昆虫是从2018年至2020年在Velho港JaciParaná农村社区的鸡舍中收集的，朗多尼亚,巴西,同时对公鸡进行血液喂养。将蚊子随机分组在池中,从池中浸渍头部和胸部,并通过定量逆转录聚合酶链反应(RT-qPCR)检查MAYV的存在。阳性池用于感染C6/36细胞系，在感染后的不同日子，通过RT-qPCR对感染细胞的上清液进行病毒检测。
结果：总共测试了183池雌性蚊子，其中18％对MAYV呈阳性；来自接种到C6/36细胞中的昆虫池的一些样品在感染后3至7天之间显示出体外增殖能力。
结论：这是Ma的第一份报告。被MAYV自然感染的肱骨蚊子，表明这些载体可能是这种虫媒病毒的潜在传播因子。
