PDF(Pubmed)
Abstract：
Prenatal alcohol exposure (PAE) refers to fetal exposure to alcohol during pregnancy through placental barrier transfer from maternal blood. The postnatal outcomes of PAE differ among exposed individuals and range from overt (serious) alcohol-related behavioral and neurophysiological impairments to covert (silenced) symptoms. The aims of the present investigation were to assess the postnatal neurobehavioral disturbances, particularly, motor coordination and sensory-motor function in mice with PAE. Female mice with positive vaginal plugs were divided into three groups: group 1: Et + Pyr: received two i.p injections of ethanol (1 g/kg) followed by pyrazole (100 mg/kg). Group 2: Pyr: received an i.p injection of pyrazole (100 mg/kg). Group 3: C: of saline controls received, in equal volume, saline solution (NaCl 0.9%). After birth, mice pups were weighed and subjected to behavioral tests for motor function screening using the motor ambulation test, cliff aversion, surface righting, and negative geotaxis, while at the adult stage, mice were subjected to the open field, rotarod, parallel bars, and static rods tests. Our data show an obvious decrement of body weight from the first post-natal day (P1) and continues over the adult stage. This was accompanied by an obvious impaired sensory-motor function which was maintained even at the adult stage with alteration of the locomotor and coordination abilities. The current data demonstrate the powerful neurotoxic effect of prenatal ethanol exposure on the sensory-motor and coordination functions, leading to suppose possible structural and/or functional neuronal disturbances, particularly the locomotor network.
摘要：
产前酒精暴露（PAE）是指胎儿在怀孕期间通过胎盘屏障从母体血液转移而暴露于酒精。PAE的产后结局因暴露个体而异，范围从明显（严重）与酒精相关的行为和神经生理障碍到隐蔽（沉默）症状。本研究的目的是评估出生后的神经行为障碍，特别是,PAE小鼠的运动协调和感觉运动功能。将具有阳性阴道塞的雌性小鼠分成三组:组1:Et+Pyr:接受两次i.p注射乙醇(lg/kg),随后接受吡唑(100mg/kg)。组2:Pyr:接受静脉注射吡唑(100mg/kg)。第3组：C：接受的盐水对照组，在相等的体积，盐溶液（NaCl0.9%）。出生后,对小鼠幼崽进行称重，并进行行为测试，以使用运动行走测试进行运动功能筛查，悬崖厌恶,表面扶正,和负地轴，在成人阶段，将小鼠置于开放的田野中，旋转杆,平行杆,和静态棒测试。我们的数据显示，从出生后的第一天（P1）开始，体重明显下降，并持续到成年阶段。伴随着明显的感觉运动功能受损，即使在成年阶段也能保持运动和协调能力的改变。目前的数据表明，产前乙醇暴露对感觉运动和协调功能具有强大的神经毒性作用，导致假设可能的结构和/或功能神经元紊乱，特别是运动网络。
