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Abstract：
Single-cell sequencing has become one of the most used techniques across the wide field of biology. It has enabled researchers to investigate the whole transcriptome at the cellular level across tissues, which unlocks numerous potentials for basic and applied studies in future diagnosis and therapy. Here, we review the impact of single-cell RNA sequencing, as the prominent single-cell technique, in pancreatic biology and cancer. We discuss the most recent findings about pancreatic physiology and pathophysiology owing to this technological advancement in the past few years. Using single-cell RNA sequencing, researchers have been able to discover cellular heterogeneity across healthy cell types, as well as cancer tissues of the pancreas. We will discuss the new immunological targets and new molecular mechanisms of progression in the microenvironment of pancreatic cancer studied using single-cell RNA sequencing. The scope is not limited to cancer tissues, and we cover novel developmental, evolutionary, physiological, and heterogenic insights that have also been achieved recently for pancreatic tissues. We cover all biological insights derived from the single-cell RNA sequencing data, discuss the corresponding pros and cons, and finally, conclude how future research can move better by utilizing single-cell analysis for pancreatic biology.
摘要：
单细胞测序已成为生物学广泛领域中最常用的技术之一。它使研究人员能够在整个组织的细胞水平上研究整个转录组，这为未来的诊断和治疗中的基础和应用研究释放了许多潜力。这里,我们回顾了单细胞RNA测序的影响，作为突出的单细胞技术，胰腺生物学和癌症。由于过去几年的技术进步，我们讨论了有关胰腺生理学和病理生理学的最新发现。使用单细胞RNA测序，研究人员已经能够发现健康细胞类型的细胞异质性，以及胰腺癌组织。我们将讨论使用单细胞RNA测序研究的胰腺癌微环境中的新免疫靶标和新的分子机制。范围不仅限于癌组织，我们涵盖了新的发展，进化，生理,以及最近在胰腺组织中也取得的异源性见解。我们涵盖了来自单细胞RNA测序数据的所有生物学见解，讨论相应的利弊，最后，总结未来的研究如何利用单细胞分析胰腺生物学更好地发展。
