PDF(Pubmed)
Abstract：
Melanoma is a dangerous type of skin cancer sometimes treated with radiotherapy. However, it induces damage to the surrounding healthy tissue and possibly further away areas. Therefore, it is necessary to give a lower dose to the patient with targeted therapy. In this study, the radio-sensitising effect of gold-coated iron oxide nanoparticles on electron beam radiotherapy of a melanoma tumour with magnetic targeting in a mouse model was investigated. Gold-coated iron oxide nanoparticles were prepared in a steady procedure. The melanoma tumour model was induced in mice. Animals were divided into five groups: (1) normal; (2) melanoma; (3) gold-coated iron oxide nanoparticles alone; (4) electron beam radiotherapy; (5) electron beam radiotherapy plus gold-coated iron oxide nanoparticles. The magnet was placed on the tumour site for 2 h. The tumours were then exposed to 6 MeV electron beam radiotherapy for a dose of 8 Gy. Inductively coupled plasma optical emission spectrometry test, hematoxylin and eosin staining, and enzyme-linked immunosorbent assay blood test were also performed. Gold-coated iron oxide nanoparticles with magnetic targeting before electron beam radiotherapy reduced the growth of the tumour compared to the control group. Blood tests did not show any significant toxicity. Deposition of nanoparticles was more in the tumour and spleen tissue and to a lesser extent in the liver, kidney, and lung tissues. The synergistic effect of nanoparticles administered by the intraperitoneal route and then concentrated into the tumour area by application of an external permanent magnet, before delivery of the electron beam radiotherapy improved the overall cancer treatment outcome and prevented metal distribution side effects.
摘要：
黑色素瘤是一种危险的皮肤癌，有时会接受放射治疗。然而,它对周围的健康组织和可能更远的区域造成损害。因此,有必要对患者给予较低剂量的靶向治疗。在这项研究中,在小鼠模型中研究了金包覆的氧化铁纳米颗粒对具有磁靶向的黑色素瘤肿瘤的电子束放射治疗的放射增敏作用。在稳定的过程中制备了金包覆的氧化铁纳米颗粒。在小鼠中诱导黑素瘤肿瘤模型。动物分为五组：（1）正常；（2）黑色素瘤；（3）单独的金包覆的氧化铁纳米颗粒；（4）电子束放射治疗；（5）电子束放射治疗加金包覆的氧化铁纳米颗粒。将磁体放置在肿瘤部位上2小时。然后将肿瘤暴露于6MeV电子束放射治疗,剂量为8Gy。电感耦合等离子体发射光谱测试,苏木精和伊红染色，并进行了酶联免疫吸附试验血液检测。与对照组相比，在电子束放疗之前具有磁性靶向的金包覆的氧化铁纳米颗粒减少了肿瘤的生长。血液测试未显示任何明显的毒性。纳米颗粒的沉积更多的是在肿瘤和脾组织，在肝脏的程度较小，肾,和肺组织。通过腹膜内途径给药的纳米颗粒的协同作用，然后通过应用外部永磁体集中到肿瘤区域，在实施电子束放疗前,可改善整体癌症治疗结果,并防止金属分布副作用.
