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Abstract：
OBJECTIVE: Cryofibrinogens (CFs) and cryoglobulins (CGs) are cryoproteins responsible for obstructive vasculopathy and vasculitis. The aim of this study was to compare the characteristics of CF and CG, and to define the conditions of their association.
RESULTS: This retrospective study was conducted at the Lyon University Hospitals, and included patients with at least one sample tested for CF and/or CG between September 2013 and April 2021. Serum and plasma samples were analyzed in very strict conditions of temperature. After cold precipitation, CF and CG were characterized and quantified in the cryoprecipitates. CRP and plasma fibrinogen levels were also investigated. Over this 7-year period, 1,712 samples for CF detection and 25,650 samples for CG detection were sent to the laboratory. Simultaneous testing of CF and CG was performed in 1,453/1,712 samples (85%). CF was less often positive than CG (8.3 vs. 13.5%, p < 0.0001). In positive CF samples, CG was associated in 28.9% of cases. In CF, fibrinogen was associated with fibronectin in 98/142 (69%) samples, especially in highly concentrated CF. CF concentration was independent of C-reactive protein and plasma fibrinogen concentrations.
CONCLUSIONS: The simultaneous detection of CF and CG is essential for the diagnosis of vasculitis or thromboembolic events and their treatment.
RESULTS: This retrospective study was conducted at the Lyon University Hospitals, and included patients with at least one sample tested for CF and/or CG between September 2013 and April 2021. Serum and plasma samples were analyzed in very strict conditions of temperature. After cold precipitation, CF and CG were characterized and quantified in the cryoprecipitates. CRP and plasma fibrinogen levels were also investigated. Over this 7-year period, 1,712 samples for CF detection and 25,650 samples for CG detection were sent to the laboratory. Simultaneous testing of CF and CG was performed in 1,453/1,712 samples (85%). CF was less often positive than CG (8.3 vs. 13.5%, p < 0.0001). In positive CF samples, CG was associated in 28.9% of cases. In CF, fibrinogen was associated with fibronectin in 98/142 (69%) samples, especially in highly concentrated CF. CF concentration was independent of C-reactive protein and plasma fibrinogen concentrations.
CONCLUSIONS: The simultaneous detection of CF and CG is essential for the diagnosis of vasculitis or thromboembolic events and their treatment.
摘要：
目的：冷冻纤维蛋白原（CFs）和冷球蛋白（CGs）是引起阻塞性血管病变和血管炎的冷冻蛋白。本研究的目的是比较CF和CG的特征，并定义他们的联系条件。
结果：这项回顾性研究是在里昂大学医院进行的，纳入了2013年9月至2021年4月期间至少有一个CF和/或CG样本的患者.在非常严格的温度条件下分析血清和血浆样品。经过寒冷的降水，在冷沉淀物中表征和定量CF和CG。还研究了CRP和血浆纤维蛋白原水平。在这7年的时间里,1,712个用于CF检测的样品和25,650个用于CG检测的样品被送到实验室。在1,453/1,712个样品(85%)中进行CF和CG的同时测试。CF的阳性程度低于CG（8.3vs.13.5%,p<0.0001)。在正CF样本中，28.9%的病例与CG相关。在CF中，在98/142（69％）样品中，纤维蛋白原与纤连蛋白相关，特别是在高浓度的CF。CF浓度与C反应蛋白和血浆纤维蛋白原浓度无关。
结论：同时检测CF和CG对于血管炎或血栓栓塞事件的诊断及其治疗至关重要。
结果：这项回顾性研究是在里昂大学医院进行的，纳入了2013年9月至2021年4月期间至少有一个CF和/或CG样本的患者.在非常严格的温度条件下分析血清和血浆样品。经过寒冷的降水，在冷沉淀物中表征和定量CF和CG。还研究了CRP和血浆纤维蛋白原水平。在这7年的时间里,1,712个用于CF检测的样品和25,650个用于CG检测的样品被送到实验室。在1,453/1,712个样品(85%)中进行CF和CG的同时测试。CF的阳性程度低于CG（8.3vs.13.5%,p<0.0001)。在正CF样本中，28.9%的病例与CG相关。在CF中，在98/142（69％）样品中，纤维蛋白原与纤连蛋白相关，特别是在高浓度的CF。CF浓度与C反应蛋白和血浆纤维蛋白原浓度无关。
结论：同时检测CF和CG对于血管炎或血栓栓塞事件的诊断及其治疗至关重要。
