PDF(Pubmed)
Abstract：
BACKGROUND: We report large biofilm structures that covered almost the entirety of the lumen and surface of double-J stents in two postrenal transplant patients, with no development of urinary tract infection. Biofilm bacteria of one patient were integrated by coccus in a net structure, whereas overlapping cells of bacilli were present in the other patient. To the best of our knowledge, this is the first time that high-quality images of the architecture of noncrystalline biofilms have been found inside double-J stents from long-term stenting in renal transplant recipients.
METHODS: Two renal transplant recipients, a 34-year-old male and a 39-year-old female of Mexican-Mestizo origin, who underwent a first renal transplant and lost it due to allograft failure, had a second transplant. Two months after the surgical procedure, double-J stents were removed and analyzed using scanning electron microscopy (SEM). None of the patients had an antecedent of UTI, and none developed UTI after urinary device removal. There were no reports of injuries, encrustation, or discomfort caused by these devices.
CONCLUSIONS: The bacterial biofilm inside the J stent from long-term stenting in renal transplant recipients was mainly concentrated on unique bacteria. Biofilm structures from the outside and inside of stents do not have crystalline phases. Internal biofilms may represent a high number of bacteria in the double-J stent, in the absence of crystals.
METHODS: Two renal transplant recipients, a 34-year-old male and a 39-year-old female of Mexican-Mestizo origin, who underwent a first renal transplant and lost it due to allograft failure, had a second transplant. Two months after the surgical procedure, double-J stents were removed and analyzed using scanning electron microscopy (SEM). None of the patients had an antecedent of UTI, and none developed UTI after urinary device removal. There were no reports of injuries, encrustation, or discomfort caused by these devices.
CONCLUSIONS: The bacterial biofilm inside the J stent from long-term stenting in renal transplant recipients was mainly concentrated on unique bacteria. Biofilm structures from the outside and inside of stents do not have crystalline phases. Internal biofilms may represent a high number of bacteria in the double-J stent, in the absence of crystals.
摘要：
背景：我们报告了两名肾移植后患者的大型生物膜结构，其几乎覆盖了双J支架的整个管腔和表面，没有尿路感染的发展。一名患者的生物膜细菌被球菌整合成网状结构，而其他患者存在重叠的杆菌细胞。据我们所知,这是首次在肾移植受者长期支架置入的双J支架内发现高质量的非晶体生物膜结构图像.
方法：两名肾移植受者，一名34岁的男性和一名39岁的女性来自墨西哥混血儿，他接受了第一次肾移植，但由于同种异体移植失败而失去了移植，进行了第二次移植。手术后两个月,取出双J支架并使用扫描电子显微镜（SEM）进行分析。没有一个病人有UTI的前身,尿路装置取出后无尿路感染。没有受伤报告,结壳,或由这些设备引起的不适。
结论：肾移植受者长期支架置入后J支架内的细菌生物膜主要集中在独特的细菌上。来自支架外部和内部的生物膜结构不具有结晶相。内部生物膜可能代表了双J支架中大量的细菌，在没有晶体的情况下。
方法：两名肾移植受者，一名34岁的男性和一名39岁的女性来自墨西哥混血儿，他接受了第一次肾移植，但由于同种异体移植失败而失去了移植，进行了第二次移植。手术后两个月,取出双J支架并使用扫描电子显微镜（SEM）进行分析。没有一个病人有UTI的前身,尿路装置取出后无尿路感染。没有受伤报告,结壳,或由这些设备引起的不适。
结论：肾移植受者长期支架置入后J支架内的细菌生物膜主要集中在独特的细菌上。来自支架外部和内部的生物膜结构不具有结晶相。内部生物膜可能代表了双J支架中大量的细菌，在没有晶体的情况下。
