Abstract：
OBJECTIVE: In-vivo studies of the bioavailability of major components of the tumor necrosis factor alpha (TNFα) biosystem inside the gestational sac during embryogenesis have not been reported. We sought to determine the concentration of TNFα, soluble (s) TNFα receptors (sTNFR1, sTNFR2), and RANTES in the primate extraembryonic celomic fluid (ECF).
METHODS: A validated timed-pregnant baboon animal model (N: 10) for experimental research in pregnancy was used to collect paired maternal blood and ECF samples in ongoing pregnancies. The concentrations (pg/dL) of TNFα, sTNFR1, sTNFR2, and RANTES were then determined by ELISA immunoassays.
RESULTS: All animals delivered at term healthy newborns. The differential concentration of TNFα, sTNFR1, sTNFR2, and RANTES between the maternal plasma and the ECF could be determined with ratios for TNFα (5.4), sTNFR2 (1.85) and RANTES (3.59) that contrasted with that of sTNFR1 (0.07), which favored the gestational sac compartment. No significant correlations were noted between maternal plasma and ECF TNFR1, sTNFR2 and RANTES. There was a trend for a correlation between TNFα in maternal plasma and ECF (R=0.74; p=0.07).
CONCLUSIONS: We report the physiological concentrations of TNFα, sTNFR1, sTNFR2, and RANTES in extraembryonic celomic fluid during embryogenesis in primates.
METHODS: A validated timed-pregnant baboon animal model (N: 10) for experimental research in pregnancy was used to collect paired maternal blood and ECF samples in ongoing pregnancies. The concentrations (pg/dL) of TNFα, sTNFR1, sTNFR2, and RANTES were then determined by ELISA immunoassays.
RESULTS: All animals delivered at term healthy newborns. The differential concentration of TNFα, sTNFR1, sTNFR2, and RANTES between the maternal plasma and the ECF could be determined with ratios for TNFα (5.4), sTNFR2 (1.85) and RANTES (3.59) that contrasted with that of sTNFR1 (0.07), which favored the gestational sac compartment. No significant correlations were noted between maternal plasma and ECF TNFR1, sTNFR2 and RANTES. There was a trend for a correlation between TNFα in maternal plasma and ECF (R=0.74; p=0.07).
CONCLUSIONS: We report the physiological concentrations of TNFα, sTNFR1, sTNFR2, and RANTES in extraembryonic celomic fluid during embryogenesis in primates.
摘要：
目的：在胚胎发生过程中，孕囊内肿瘤坏死因子α（TNFα）生物系统主要成分的生物利用度的体内研究尚未报道。我们试图确定TNFα的浓度，可溶性TNFα受体（sTNFR1，sTNFR2），和RANTES在灵长类动物胚外细胞液（ECF）中。
方法：用于妊娠实验研究的经过验证的定时怀孕的狒狒动物模型（N：10）用于在正在进行的妊娠中收集配对的母体血液和ECF样本。TNFα的浓度（pg/dL），然后通过ELISA免疫测定法测定sTNFR1、sTNFR2和RANTES。
结果：所有动物均在足月健康新生儿分娩。TNFα的浓度差异，母体血浆和ECF之间的sTNFR1，sTNFR2和RANTES可以用TNFα的比率确定（5.4），sTNFR2(1.85)和RANTES(3.59)与sTNFR1(0.07)的对比，这有利于妊娠囊室。母体血浆与ECFTNFR1、sTNFR2和RANTES之间没有显著相关性。母体血浆中的TNFα与ECF之间存在相关性的趋势（R=0.74；p=0.07）。
结论：我们报告了TNFα的生理浓度，灵长类动物胚胎发生过程中胚外细胞液中的sTNFR1、sTNFR2和RANTES。
方法：用于妊娠实验研究的经过验证的定时怀孕的狒狒动物模型（N：10）用于在正在进行的妊娠中收集配对的母体血液和ECF样本。TNFα的浓度（pg/dL），然后通过ELISA免疫测定法测定sTNFR1、sTNFR2和RANTES。
结果：所有动物均在足月健康新生儿分娩。TNFα的浓度差异，母体血浆和ECF之间的sTNFR1，sTNFR2和RANTES可以用TNFα的比率确定（5.4），sTNFR2(1.85)和RANTES(3.59)与sTNFR1(0.07)的对比，这有利于妊娠囊室。母体血浆与ECFTNFR1、sTNFR2和RANTES之间没有显著相关性。母体血浆中的TNFα与ECF之间存在相关性的趋势（R=0.74；p=0.07）。
结论：我们报告了TNFα的生理浓度，灵长类动物胚胎发生过程中胚外细胞液中的sTNFR1、sTNFR2和RANTES。
