UNASSIGNED: To understand the role of MSTN and FGF5 in reproductive performance and visceral organs, this study evaluated the ejaculation amount, semen pH, sperm motility, sperm density, acrosome integrity, rate of teratosperm, and seminal plasma biochemical indicators in adult MF-/- rams. We also compared the overall morphology, head, head-neck junction, middle segment and the transection of middle segment of spermatozoa between wildtype (WT) and MF-/- rams.
UNASSIGNED: Our results showed that the seminal plasma biochemical indicators, sperm structure and all sperm indicators were normal, and the fertilization rate also has no significant difference between WT and MF-/- rams, indicating that the MF-/- mutation did not affect the reproductive performance of sheep. Additional analysis evaluated the histomorphology of the visceral organs, digestive system and reproductive system of MF+/- sheep, the F1 generation of MF-/-, at the age of 12 months. There was an increased spleen index, but no significant differences in the organ indexes of heart, liver, lung, kidney and stomach, and no obvious differences in the histomorphology of visceral organs, digestive system and reproductive system in MF+/- compared with WT sheep. No MF+/- sheep were observed to have any pathological features.
UNASSIGNED: In summary, the MSTN and FGF5 double-knockout did not affect reproductive performance, visceral organs and digestive system in sheep except for differences previously observed in muscle and fat. The current data provide a reference for further elucidating the application of MSTN and FGF5 double-knockout sheep.
■为了了解MSTN和FGF5在生殖性能和内脏器官中的作用,这项研究评估了射精量,精液pH值,精子运动性,精子密度,顶体完整性,畸形精子率,和成年MF-/-公羊的精浆生化指标。我们还比较了整体形态,头部,头颈部连接处,野生型(WT)和MF-/-公羊之间的精子中段和中段的横切。
■我们的结果表明,精浆生化指标,精子结构和所有精子指标均正常,WT和MF-/-公羊的受精率也没有显着差异,说明MF-/-突变不影响绵羊的繁殖性能。其他分析评估了内脏器官的组织形态学,MF+/-绵羊的消化系统和生殖系统,F1代MF-/-,在12个月的年龄。脾脏指数升高,但是心脏的器官指数没有显着差异,肝脏,肺,肾和胃,内脏器官的组织形态学没有明显差异,MF+/-与WT绵羊的消化系统和生殖系统比较。没有观察到MF+/-绵羊具有任何病理特征。
■总之,MSTN和FGF5双敲除不影响繁殖性能,绵羊的内脏器官和消化系统,除了以前在肌肉和脂肪中观察到的差异。目前的数据为进一步阐明MSTN和FGF5双敲除绵羊的应用提供了参考。