关键词: COI Cloning Sequencing Species identification Substitution

来  源:   DOI:10.4081/ijfs.2023.11074   PDF(Pubmed)

Abstract:
Due to the close relationship between pets and humans, pet owners are highly invested in proper diets for their pets. Even though pet food mislabeling is concerning, there are few studies on this topic. This study investigated pet food mislabeling in South Korea\'s market based on DNA barcoding. In total, 10 pet food products were purchased, and 200 sequences of the partial Cytochrome c oxidase subunit 1 (COI) gene were generated from clones of the samples. The obtained sequences were compared to available public databases to identify species present in the ingredients. The data analyses showed that the labeled species were consistent with species detected by COI sequences in 6 of the products. However, the expected species were not detected in 4 products, revealing possible mislabeling in these samples. Our findings indicated that DNA barcoding might represent a promising tool to detect pet food mislabeling.
摘要:
由于宠物与人类的密切关系,宠物主人高度投资于适当的饮食为他们的宠物。尽管宠物食品贴错标签令人担忧,关于这个主题的研究很少。这项研究调查了基于DNA条形码的韩国市场上的宠物食品错误标记。总的来说,购买了10种宠物食品,从样品的克隆中产生200个部分细胞色素C氧化酶亚基1(COI)基因的序列。将获得的序列与可用的公共数据库进行比较以鉴定成分中存在的物种。数据分析表明,标记的物种与6种产品中通过COI序列检测到的物种一致。然而,在4种产品中未检测到预期的物种,揭示这些样品中可能的错误标签。我们的发现表明,DNA条形码可能是检测宠物食品错误标记的有前途的工具。
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