Abstract:
Calcification is a well-known process of calcium phosphate mineralization observed in intraocular lenses. Despite the many works conducted in this field, there is no strict explanation of the mechanisms of this process. In order to better understand the phenomenon, i.e., the mechanisms and structural conditions that promote calcification, any research observations should be conducted under conditions that best reflect those of the human eye. Taking into account the specific anatomy and physicochemical conditions of the human eye, the problem under discussion becomes difficult to solve in vitro. In the present study, calcium phosphates formed under conditions similar to those in the human eye were characterized using SEM/EDS and infrared spectroscopy. Conducted study showed the formation of white spherical precipitates, which are unstable when extracted from solution. Such precipitates were characteristic of solutions containing 1.5-3.0 mM2 of solutes. Elemental analysis showed a Ca/P ratio of 1.64-1.65, which is similar to the ratio for hydroxyapatite (1.67). Chemical structure analysis revealed the presence of broad bending and stretching bands at 475-830 cm-1 and 880-1250 cm-1, respectively, which are characteristic of PO43- groups in apatite calcium phosphates. In further analysis involving numerical fitting the bands corresponding to apatitic PO43- and indicating the presence of calcium phosphates hydration were found. The results allow the selection of immersion media for further studies involving the incubation of hydrogel intraocular lenses.
摘要:
钙化是在人工晶状体中观察到的众所周知的磷酸钙矿化过程。尽管在这一领域进行了许多工作,这个过程的机制没有严格的解释。为了更好地理解这一现象,即,促进钙化的机制和结构条件,任何研究观察都应该在最能反映人眼的条件下进行。考虑到人眼的特定解剖学和物理化学条件,正在讨论的问题变得很难在体外解决。在本研究中,使用SEM/EDS和红外光谱表征在与人眼相似的条件下形成的磷酸钙。进行的研究表明,白色球形沉淀物的形成,从溶液中提取时不稳定。这种沉淀物是含有1.5-3.0mM2溶质的溶液的特征。元素分析显示Ca/P比为1.64-1.65,其类似于羟基磷灰石的比率(1.67)。化学结构分析表明,分别在475-830cm-1和880-1250cm-1处存在宽的弯曲和拉伸带,这是磷灰石磷酸钙中PO43-基团的特征。在涉及数值拟合的进一步分析中,发现了对应于磷灰石PO43-并表明存在磷酸钙水合的条带。结果允许选择浸没介质用于涉及水凝胶眼内透镜的孵育的进一步研究。