PDF(Pubmed)
Abstract：
Introduction: Bladder cancer (BLCA) is one of the most common malignancies in the urinary system with a poor prognosis and high treatment costs. Identifying potential prognostic biomarkers is significant for exploring new therapeutic and predictive targets of BLCA. Methods: In this study, we screened differentially expressed genes using the GSE37815 dataset. We then performed a weighted gene co-expression network analysis (WGCNA) to identify the genes correlated with the histologic grade and T stage of BLCA using the GSE32548 dataset. Subsequently, Kaplan Meier survival analysis and Cox regression were used to further identify prognosis-related hub genes using the datasets GSE13507 and TCGA-BLCA. Moreover, we detected the expression of the hub genes in 35 paired samples, including BLCA and paracancerous tissue, from the Shantou Central Hospital by qRT-polymerase chain reaction. Results: This study showed that Anillin (ANLN) and Abnormal spindle-like microcephaly-associated gene (ASPM) were prognostic biomarkers for BLCA. High expression of ANLN and ASPM was associated with poor overall survival.The qRT-PCR results revealed that ANLN and ASPM genes were upregulated in BLCA, and there was a correlation between the expression of ANLN and ASPM in cancer tissues and paracancerous tissue. Additionally, the increasing multiples in the ANLN gene was obvious in high-grade BLCA. Discussion: In summary, this preliminary exploration indicated a correlation between ANLN and ASPM expression. These two genes, serving as the risk factors for BLCA progression, might be promising targets to improve the occurrence and progression of BLCA.
摘要：
简介:膀胱癌(BLCA)是泌尿系统最常见的恶性肿瘤之一,预后差,治疗费用高。识别潜在的预后生物标志物对于探索BLCA的新治疗和预测靶标具有重要意义。方法:在本研究中,我们使用GSE37815数据集筛选了差异表达基因.然后，我们使用GSE32548数据集进行加权基因共表达网络分析（WGCNA）以鉴定与BLCA的组织学分级和T期相关的基因。随后，使用数据集GSE13507和TCGA-BLCA,使用Kaplan-Meier生存分析和Cox回归进一步鉴定预后相关中枢基因。此外，我们在35个配对样本中检测到hub基因的表达，包括BLCA和癌旁组织，来自汕头市中心医院的qRT-聚合酶链反应。结果：这项研究表明，Anillin（ANLN）和异常纺锤形小头畸形相关基因（ASPM）是BLCA的预后生物标志物。ANLN和ASPM的高表达与低总生存率相关。qRT-PCR结果显示ANLN和ASPM基因在BLCA中表达上调,ANLN和ASPM在癌组织和癌旁组织中的表达存在相关性。此外,在高级别BLCA中，ANLN基因的倍数增加明显。讨论：总之，这一初步探索表明ANLN和ASPM表达之间存在相关性。这两个基因,作为BLCA进展的危险因素，可能是改善BLCA发生和进展的有希望的目标。
