Abstract：
Gastrointestinal motility disorder (GMD) is a disease that causes digestive problems due to inhibition of the movement of the gastrointestinal tract and is one of the diseases that reduce the quality of life of modern people. Smilacis Glabrae Rhixoma (SGR) is a traditional herbal medicine for many diseases and is sometimes prescribed to improve digestion. As a network pharmacological approach, we searched the TCMSP database for SGR, reviewed its constituents and target genes, and analyzed its relevance to gastrointestinal motility disorder. The effects of the SGR extract on the pacemaker activity in interstitial cells of Cajal (ICC) and gastric emptying were investigated. In addition, using the GMD mouse model through acetic acid (AA), we investigated the locomotor effect of SGR on the intestinal transit rate (ITR). As a result of network pharmacology analysis, 56 compounds out of 74 candidate compounds of SGR have targets, the number of targets is 390 targets, and there are 904 combinations. Seventeen compounds of SGR were related to GMD, and as a result of comparing the related genes with the GMD-related genes, 17 genes (active only) corresponded to both. When looking at the relationship network between GMD and SGR, it was confirmed that quercetin, resveratrol, SCN5A, TNF, and FOS were most closely related to GMD. In addition, the SGR extract regulated the pacemaker activity in ICC and recovered the delayed gastric emptying. As a result of feeding the SGR extract to AA-induced GMD mice, it was confirmed that the ITR decreased by AA was restored by the SGR extract. Through network pharmacology, it was confirmed that quercetin, resveratrol, SCN5A, TNF, and FOS were related to GMD in SGR, and these were closely related to intestinal motility. Based on these results, it is suggested that SGR in GMD restores digestion through the recovery of intestinal motility.
摘要：
胃肠动力障碍（GMD）是一种由于胃肠道运动受到抑制而引起消化问题的疾病，是降低现代人生活质量的疾病之一。SmilacisGlabraeRhixoma（SGR）是一种治疗许多疾病的传统草药，有时用于改善消化。作为一种网络药理学方法，我们在TCMSP数据库中搜索了SGR，回顾了它的成分和靶基因，并分析其与胃肠动力障碍的相关性。研究了SGR提取物对Cajal间质细胞（ICC）起搏器活性和胃排空的影响。此外,使用GMD小鼠模型通过乙酸（AA），我们研究了SGR对肠转运率（ITR）的运动作用。作为网络药理学分析的结果，SGR的74个候选化合物中有56个化合物具有靶标，目标数量是390个目标，有904种组合。SGR的17个化合物与GMD有关，作为比较相关基因和GMD相关基因的结果，17个基因(仅活性)对应于两者。在查看GMD和SGR之间的关系网络时，证实槲皮素,白藜芦醇,SCN5A,TNF,FOS与GMD关系最密切。此外,SGR提取物调节ICC中的起搏器活性并恢复胃排空延迟。作为给AA诱导的GMD小鼠喂食SGR提取物的结果，证实了由AA降低的ITR被SGR提取物恢复。通过网络药理学，证实槲皮素,白藜芦醇,SCN5A,TNF,FOS与SGR中的GMD有关，这些与肠道运动密切相关。基于这些结果,建议GMD中的SGR通过恢复肠动力来恢复消化。
