Abstract：
Polysaccharides with molecular weights ranging from 1.75 × 103 to 1.14 × 104 g/mol were obtained from the fruit bodies of Ganoderma lucidum. The multiple fingerprints and macrophage immunostimulatory activity of these fractions were analyzed as well as the fingerprint-activity relationship. The correlation analysis of molecular weight and immune activity demonstrated that polysaccharides with molecular weights of 4.27 × 103~5.27 × 103 and 1 × 104~1.14 × 104 g/mol were the main active fractions. Moreover, the results showed that galactose, mannose, and glucuronic acid were positively related to immunostimulatory activity. Additionally, partial least-squares regression and grey correlation degree analyses indicated that three peaks (P2, P3, P8) in the oligosaccharide fragment fingerprint significantly affected the immune activity of the polysaccharides. Hence, these ingredients associated with activity could be considered as markers to assess Ganoderma lucidum polysaccharides and their related products, and the study also provides a reference for research on the spectrum-effect relationship of polysaccharides in the future.
摘要：
从灵芝子实体中获得分子量为1.75×103至1.14×104g/mol的多糖。分析了这些组分的多重指纹图谱和巨噬细胞免疫刺激活性以及指纹-活性关系。分子量和免疫活性的相关性分析表明，分子量为4.27×103〜5.27×103和1×104〜1.14×104g/mol的多糖是主要的活性组分。此外，结果表明，半乳糖，甘露糖,葡萄糖醛酸与免疫刺激活性呈正相关。此外,偏最小二乘回归和灰色关联度分析表明，寡糖片段指纹图谱中的三个峰(P2、P3、P8)显著影响多糖的免疫活性。因此，这些与活性相关的成分可以被认为是评估灵芝多糖及其相关产品的标记，该研究也为今后多糖的谱效关系研究提供了参考。
