Abstract：
The perfluoroalkyl substances (PFAS) represent a persistent class of synthetic chemicals that spread in the environment as a result of industrialization. Due to their bioaccumulative and endocrine disruption implications, these chemicals can affect food quality and human health, respectively. In the present study, the bioconcentration and biotransformation of perfluorooctanoic acid (PFOA) in common carp (Cyprinus carpio) were evaluated in a biphasic system (exposure and depuration). Carp were continuously exposed, under laboratory conditions, to 10 (Experiment 1) and 100 (Experiment 2) µg/L PFOA for 14 weeks, followed by a wash out period of 3 weeks. Fish organs and tissues were collected at 8, 12, 14 weeks of exposure and at week 17, after the depuration period. The results obtained from the LC-MS/MS analysis showed the presence of PFOA in all studied organs. The highest values of PFOA were identified in the gallbladder (up to 2572 ng/g d.w.) in Experiment 1 and in the gallbladder (up to 18,640 ng/g d.w.) and kidneys (up to 13,581 ng/g d.w.) in Experiment 2. The average BCF varied between 13.4 and 158 L/Kg in Experiment 1 and between 5.97 and 80.3 L/Kg in Experiment 2. Four biotransformation products were identified and quantified in all organs, namely: PFBA, PFPeA, PFHxA, and PFHpA. PFBA was proven to be the dominant biotransformation product, with the highest values being determined after 8 weeks of exposure in the kidney, gallbladder, brain, liver, and gonads in both experiments. Because freshwater fish are an important food resource for the human diet, the present study showed the fishes\' capacity to accumulate perfluoroalkyl substances and their metabolites. The study revealed the necessity of monitoring and risk studies of new and modern synthetic chemicals in aquatic resources.
摘要：
全氟烷基物质（PFAS）代表了一类持久性的合成化学品，由于工业化而在环境中传播。由于它们的生物蓄积性和内分泌干扰的影响，这些化学物质会影响食品质量和人类健康，分别。在本研究中,在双相系统（暴露和净化）中评估了鲤鱼（Cyprinuscarpio）中全氟辛酸（PFOA）的生物浓缩和生物转化。鲤鱼不断暴露，在实验室条件下，至10（实验1）和100（实验2）µg/LPFOA，持续14周，然后是3周的清洗期。在暴露8、12、14周和净化期后第17周收集鱼器官和组织。从LC-MS/MS分析获得的结果显示在所有研究的器官中存在PFOA。实验1中的胆囊（高达2572ng/gd.w.）和胆囊（高达18,640ng/gd.w.）和肾脏（高达13,581ng/gd.w.）中PFOA的最高值。实验1中平均BCF在13.4和158L/Kg之间变化,实验2中平均BCF在5.97和80.3L/Kg之间变化。在所有器官中鉴定并定量了四种生物转化产物，即:PFBA,PFPeA,PFHxA,和PFHpa。PFBA被证明是主要的生物转化产物，在肾脏暴露8周后确定的最高值，胆囊,大脑,肝脏,和性腺在两个实验中。因为淡水鱼是人类饮食的重要食物资源，本研究显示了鱼类积累全氟烷基物质及其代谢物的能力。该研究揭示了对水生资源中新型和现代合成化学物质进行监测和风险研究的必要性。
