PDF(Pubmed)
Abstract：
Heavy metal-associated proteins (HMPs) participate in heavy metal detoxification. Although HMPs have been identified in several plants, no studies to date have identified the HMPs in Brassica rapa (B. rapa). Here, we identified 85 potential HMPs in B. rapa by bioinformatic methods. The promoters of the identified genes contain many elements associated with stress responses, including response to abscisic acid, low-temperature, and methyl jasmonate. The expression levels of BrHMP14, BrHMP16, BrHMP32, BrHMP41, and BrHMP42 were upregulated under Cu2+, Cd2+, Zn2+, and Pb2+ stresses. BrHMP06, BrHMP30, and BrHMP41 were also significantly upregulated after drought treatment. The transcripts of BrHMP06 and BrHMP11 increased mostly under cold stress. After applying salt stress, the expression of BrHMP02, BrHMP16, and BrHMP78 was induced. We observed increased BrHMP36 expression during the self-incompatibility (SI) response and decreased expression in the compatible pollination (CP) response during pollen-stigma interactions. These changes in expression suggest functions for these genes in HMPs include participating in heavy metal transport, detoxification, and response to abiotic stresses, with the potential for functions in sexual reproduction. We found potential co-functional partners of these key players by protein-protein interaction (PPI) analysis and found that some of the predicted protein partners are known to be involved in corresponding stress responses. Finally, phosphorylation investigation revealed many phosphorylation sites in BrHMPs, suggesting post-translational modification may occur during the BrHMP-mediated stress response. This comprehensive analysis provides important clues for the study of the molecular mechanisms of BrHMP genes in B. rapa, especially for abiotic stress and pollen-stigma interactions.
摘要：
重金属相关蛋白(HMPs)参与重金属解毒。尽管已经在几种植物中发现了HMP，迄今为止，尚无研究确定甘蓝型油菜中的HMPs(B.rapa）。这里,我们通过生物信息学方法鉴定了85种潜在的HMPs。鉴定基因的启动子含有许多与应激反应相关的元件，包括对脱落酸的反应，低温,还有茉莉酸甲酯.BrHMP14、BrHMP16、BrHMP32、BrHMP41和BrHMP42在Cu2+下表达上调,Cd2+,Zn2+,和Pb2+应力。BrHMP06、BrHMP30和BrHMP41在干旱处理后也显著上调。在冷胁迫下，BrHMP06和BrHMP11的转录本主要增加。施加盐胁迫后，BrHMP02、BrHMP16和BrHMP78的表达被诱导。我们观察到在花粉-柱头相互作用期间，自交不亲和（SI）反应期间BrHMP36表达增加，而相容授粉（CP）反应中的表达减少。这些表达的变化表明这些基因在HMPs中的功能包括参与重金属运输，排毒,以及对非生物胁迫的反应，具有有性生殖功能的潜力。我们通过蛋白质-蛋白质相互作用（PPI）分析发现了这些关键参与者的潜在共同功能伴侣，并发现已知一些预测的蛋白质伴侣参与相应的应激反应。最后,磷酸化研究揭示了BrHMPs中的许多磷酸化位点，提示翻译后修饰可能发生在BrHMP介导的应激反应过程中。这种综合分析为研究BrHMP基因在B.rapa中的分子机制提供了重要线索，特别是对于非生物胁迫和花粉-柱头相互作用。
