Abstract：
Pheochromocytoma (PCC), a rare tumor, often develops distant metastases after diagnosis, delaying early intervention treatment. In order to overcome the limitations of traditional diagnostic methods, dual-targeting Surface-Enhancement Raman Scattering (SERS) cytosensor was developed to identify and detect PCC-CTCs from peripheral blood. Meta-iodobenzylguanidine (MIBG) and octreotide-2,2\',2″,2\'\'\'- (1,4,7,10 -tetraazacyclododecane-1,4,7,10-tetrayl) tetraacetic acid (DOTA) functionalized magnetic Fe3O4 and Ag-DTNB were prepared as capture probe and signal probe for SERS signal export, respectively. Ag nanocubes (AgNCs) as Raman active substrate offer an enhanced electromagnetic field, which could effectively enhance the signal intensity of DTNB and potentially realize trace analyte detection. The obtained SERS fingerprint spectroscopy possessed the characteristic of high sensitivity and resolution in the concentration range from 3.0-3.0 × 106 cells mL-1, with a detection limit of 1 cell mL-1, which laterally compensated the deficiency of scarce CTCs in peripheral blood. This work provided new insight into PCC-CTCs accurate detection.
摘要：
嗜铬细胞瘤(PCC),一种罕见的肿瘤,诊断后经常发生远处转移，延迟早期干预治疗。为了克服传统诊断方法的局限性,开发了双靶向表面增强拉曼散射（SERS）细胞传感器来识别和检测外周血中的PCC-CTC。间碘苄基胍（MIBG）和奥曲肽-2,2'，2″,制备2\'\'\'-（1,4,7,10-四氮杂环十二烷-1,4,7,10-四烷基）四乙酸（DOTA）功能化的磁性Fe3O4和Ag-DTNB作为SERS信号输出的捕获探针和信号探针，分别。Ag纳米立方体(AgNCs)作为拉曼活性基底提供增强的电磁场,能有效增强DTNB的信号强度,有可能实现痕量分析物的检测。获得的SERS指纹图谱在3.0-3.0×106个细胞mL-1的浓度范围内具有高灵敏度和分辨率的特点，检出限为1个细胞mL-1，横向补偿了外周血中稀缺CTC的缺乏。这项工作为PCC-CTC的准确检测提供了新的见解。
