PDF(Pubmed)
Abstract：
Avian infectious bronchitis (IB) is a highly contagious disease caused by infectious bronchitis virus (IBV). Vaccination is an effective approach for controlling IBV. Therefore, reliable immune monitoring for IB is critical for poultry. In this study, a novel peptide derived from S2 protein was used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of broadly cross-reactive antibodies against IBV. The peptide-based ELISA (pELISA) showed good specificity and sensitivity in detecting IBV antibodies against different serotypes. A semilogarithmic regression method for determining IBV antibody titers was also established. Antibody titers detected by pELISA and calculated with this equation were statistically similar to those evaluated by indirect fluorescence assay (IFA). Moreover, the comparison analysis showed a 96.07% compatibility between the pELISA and IDEXX ELISA. All these data demonstrate that the pELISA generated here can be as a rapid and reliable serological surveillance tool for monitoring IBV infection or vaccination.
摘要：
鸡传染性支气管炎(IB)是由传染性支气管炎病毒(IBV)引起的高度传染性疾病。疫苗接种是控制IBV的有效方法。因此,可靠的IB免疫监测对家禽至关重要。在这项研究中,一种源自S2蛋白的新型肽被用于开发酶联免疫吸附测定(ELISA),用于检测针对IBV的广泛交叉反应性抗体.基于肽的ELISA（pELISA）在检测针对不同血清型的IBV抗体方面显示出良好的特异性和敏感性。还建立了测定IBV抗体滴度的半对数回归方法。通过pELISA检测并使用该方程式计算的抗体滴度在统计学上与通过间接荧光测定（IFA）评估的抗体滴度相似。此外，比较分析显示pELISA和IDEXXELISA之间的相容性为96.07%.所有这些数据表明,此处产生的pELISA可以作为用于监测IBV感染或疫苗接种的快速和可靠的血清学监测工具。
