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Abstract：
UNASSIGNED: The aim of the present study was to observe the effect of the stroma proportion in hyperplasia nodules on the clinical symptoms of benign prostatic hyperplasia (BPH) patients and to identify the different genes and pathways in prostatic hyperplasia nodules between patients with epithelial-dominated hyperplasia (EDH) and stromal-dominated hyperplasia (SDH) nodules.
UNASSIGNED: Sixty-seven BPH patient samples underwent transurethral resection of the prostate (TURP) were collected and retrospectively analyzed. The differences in clinical parameters between the EDH and SDH groups were investigated. Collagen fiber percentage was assessed, and the correlation with clinical parameters was evaluated. mRNA sequencing in hyperplasia nodules of 8 BPH patients was performed, and differentially expressed genes (DEGs) between the EDH and SDH groups were screened. These DEGs were analyzed using GO, KEGG and PPI analysis.
UNASSIGNED: The results showed the IPSS was significantly higher in the SDH group than in the EDH group (p < 0.01). The collagen fiber percentage of BPH nodules was higher in the SDH group than in the EDH group (p < 0.05), and the collagen fiber percentage was positively correlated with the IPSS (r = 0.4058, p = 0.0007). A total of 172 DEGs were obtained, including 63 up-regulated genes and 109 down-regulated genes. GO and KEGG pathway enrichment analyses showed DEGs were mainly enriched in extracellular matrix structural constituents. The top 10 hub genes were associated to the components of extracellular matrix and fibrosis.
UNASSIGNED: These results suggested that the symptoms of BPH patients with SDH nodules may be associated with prostate fibrosis and fibrosis may be a significant contributing factor in BPH/LUTS patients with SDH nodules.
UNASSIGNED: Sixty-seven BPH patient samples underwent transurethral resection of the prostate (TURP) were collected and retrospectively analyzed. The differences in clinical parameters between the EDH and SDH groups were investigated. Collagen fiber percentage was assessed, and the correlation with clinical parameters was evaluated. mRNA sequencing in hyperplasia nodules of 8 BPH patients was performed, and differentially expressed genes (DEGs) between the EDH and SDH groups were screened. These DEGs were analyzed using GO, KEGG and PPI analysis.
UNASSIGNED: The results showed the IPSS was significantly higher in the SDH group than in the EDH group (p < 0.01). The collagen fiber percentage of BPH nodules was higher in the SDH group than in the EDH group (p < 0.05), and the collagen fiber percentage was positively correlated with the IPSS (r = 0.4058, p = 0.0007). A total of 172 DEGs were obtained, including 63 up-regulated genes and 109 down-regulated genes. GO and KEGG pathway enrichment analyses showed DEGs were mainly enriched in extracellular matrix structural constituents. The top 10 hub genes were associated to the components of extracellular matrix and fibrosis.
UNASSIGNED: These results suggested that the symptoms of BPH patients with SDH nodules may be associated with prostate fibrosis and fibrosis may be a significant contributing factor in BPH/LUTS patients with SDH nodules.
摘要：
■本研究的目的是观察增生结节中基质比例对良性前列腺增生（BPH）患者临床症状的影响，并确定前列腺增生结节中不同的基因和途径。上皮为主的增生（EDH）和基质为主的增生（SDH）结节。
■收集67例接受经尿道前列腺电切术（TURP）的BPH患者样本并进行回顾性分析。研究了EDH和SDH组之间临床参数的差异。评估胶原纤维百分比，并评估与临床参数的相关性。对8例BPH患者的增生结节进行mRNA测序,并筛选了EDH和SDH组之间的差异表达基因（DEGs）。这些DEG是用GO分析的，KEGG和PPI分析。
■结果显示，SDH组的IPSS明显高于EDH组（p<0.01）。SDH组BPH结节胶原纤维百分比高于EDH组(p<0.05),胶原纤维百分比与IPSS呈正相关(r=0.4058,p=0.0007)。总共获得了172个DEG，包括63个上调基因和109个下调基因。GO和KEGG途径富集分析显示DEGs主要富集在细胞外基质结构成分中。前10个hub基因与细胞外基质和纤维化的成分相关。
■这些结果表明，BPH伴SDH结节患者的症状可能与前列腺纤维化有关，纤维化可能是BPH/LUTS伴SDH结节患者的重要促成因素。
■收集67例接受经尿道前列腺电切术（TURP）的BPH患者样本并进行回顾性分析。研究了EDH和SDH组之间临床参数的差异。评估胶原纤维百分比，并评估与临床参数的相关性。对8例BPH患者的增生结节进行mRNA测序,并筛选了EDH和SDH组之间的差异表达基因（DEGs）。这些DEG是用GO分析的，KEGG和PPI分析。
■结果显示，SDH组的IPSS明显高于EDH组（p<0.01）。SDH组BPH结节胶原纤维百分比高于EDH组(p<0.05),胶原纤维百分比与IPSS呈正相关(r=0.4058,p=0.0007)。总共获得了172个DEG，包括63个上调基因和109个下调基因。GO和KEGG途径富集分析显示DEGs主要富集在细胞外基质结构成分中。前10个hub基因与细胞外基质和纤维化的成分相关。
■这些结果表明，BPH伴SDH结节患者的症状可能与前列腺纤维化有关，纤维化可能是BPH/LUTS伴SDH结节患者的重要促成因素。
