Abstract：
A new method for quantitative analysis of several biomarkers and pharmaceutical compounds in wastewater has been developed employing nanoflow liquid chromatography with Orbitrap mass spectrometry. An easy dilute-and-shoot approach has been used for sample preparation with a dilution factor of 5. Improved retention of ionic and highly polar compounds has been achieved by the addition of tetrabutylammonium bromide as an ion pair reagent into the final diluted sample. The new nanoflow liquid chromatography method has demonstrated low matrix effects (70%-111%), high sensitivity in terms of limits of quantification (0.005 to 0.3 μg/L), low injection volume (70 nl) and solvent consumption, and the ability to analyze diverse polar and ionic analytes within one run using a single reversed-phase nanoflow liquid chromatography column. Wastewater samples (n = 116) from the wastewater treatment plants of different cities in Latvia were analyzed using the developed method. The observed concentrations of biomarkers were in line with the literature data.
摘要：
已开发出一种采用纳米流液相色谱和Orbitrap质谱定量分析废水中几种生物标志物和药物化合物的新方法。一种简单的稀释和射击方法已用于样品制备，稀释因子为5。通过将四丁基溴化铵作为离子对试剂添加到最终稀释的样品中,已经实现了离子和高极性化合物的改进的保留。新的纳米流液相色谱法显示出低的基体效应(70%-111%)，定量限（0.005至0.3μg/L）灵敏度高，低注入体积(70nl)和溶剂消耗，以及使用单个反相纳米流液相色谱柱在一次运行中分析各种极性和离子分析物的能力。使用开发的方法分析了来自拉脱维亚不同城市污水处理厂的废水样品（n=116）。观察到的生物标志物浓度与文献数据一致。
