PDF(Pubmed)
Abstract：
The complex, dynamic environment of the human lower gastrointestinal tract is colonized by hundreds of bacterial species that impact health and performance. Ex vivo study of the functional interactions between microbial community members in conditions representative of those in the gut is an ongoing challenge. We have developed an in vitro 40-plex platform that provides an oxygen gradient to support simultaneous maintenance of microaerobic and anaerobic microbes from the gut microbiome that can aid in rapid characterization of microbial interactions and direct comparison of individual microbiome samples. In this report, we demonstrate that the platform more closely maintained the microbial diversity and composition of human donor fecal microbiome samples than strict anaerobic conditions. The oxygen gradient established in the platform allowed the stratification and subsequent sampling of diverse microbial subpopulations that colonize microaerobic and anaerobic micro-environments. With the ability to run forty samples in parallel, the platform has the potential to be used as a rapid screening tool to understand how the gut microbiome responds to environmental perturbations such as toxic compound exposure, dietary changes, or pharmaceutical treatments.
摘要：
复杂的，人体下胃肠道的动态环境被数百种影响健康和性能的细菌定植。在代表肠道中的微生物群落成员之间的功能相互作用的离体研究是一个持续的挑战。我们已经开发了一个体外40-plex平台，该平台提供了一个氧梯度，以支持同时维持来自肠道微生物组的微氧和厌氧微生物，这可以帮助快速表征微生物相互作用并直接比较单个微生物组样品。在这份报告中,我们证明，与严格的厌氧条件相比，该平台更紧密地维持了人类供体粪便微生物组样品的微生物多样性和组成。在平台中建立的氧气梯度允许对定居在微氧和厌氧微环境中的各种微生物亚群进行分层和随后的采样。能够并行运行40个样本，该平台有可能用作快速筛查工具，以了解肠道微生物组如何应对环境扰动，如有毒化合物暴露，饮食变化，或药物治疗。
