Abstract：
As onset of sepsis adversely affects the prognosis of canine pyometra, finding biomarkers that would distinguish sepsis status would be useful in the clinical management. Accordingly, we hypothesized that differential expression of endometrial transcripts and circulating concentration of certain inflammatory mediators would discriminate pyometra-led sepsis (P-sepsis+) from those of pyometra without sepsis (P-sepsis-). Bitches with pyometra (n = 52) were classified into P-sepsis+ (n = 28) and P-sepsis- (n = 24) based on vital clinical score and total leukocyte count. A group of non-pyometra bitches (n = 12) served as control. The relative fold changes in the transcripts of IL6, IL8, TNFα, IL10, PTGS2, mPGES1 and PGFS, SLPI, S100A8, S100A12 and eNOS were determined by quantitative polymerase chain reaction. Furthermore, the serum concentrations of IL6, IL8, IL10, SLPI and prostaglandin F2α metabolite (PGFM) were assayed by ELISA. The relative fold changes in S100A12 and SLPI and mean concentrations of IL6 and SLPI were significantly (p < .05) higher in P-sepsis+ than that of P-sepsis- group. Receiver operating characteristic analysis revealed that serum IL6 had a diagnostic sensitivity of 78.6% and a positive likelihood ratio (LR+) of 2.09, at a cut-off value of 15.7 pg/mL to diagnose P-sepsis+ cases. Similarly, serum SLPI had a sensitivity of 84.6% and an LR+ of 2.23, at a cut-off value of 2.0 pg/mL. It was concluded that SLPI and IL6 would serve as putative biomarkers for pyometra-led sepsis in bitches. Monitoring SLPI and IL6 would be a useful adjunct to the established haemato-biochemical parameters in customizing the treatment strategies and arriving at the decision for management of pyometra bitches with critical illness.
摘要：
由于败血症的发作会对犬子宫积脓的预后产生不利影响，发现可区分脓毒症状态的生物标志物将有助于临床治疗.因此，我们假设子宫内膜转录物的差异表达和某些炎症介质的循环浓度将区分脓宫致脓毒症(P-脓毒症+)和无脓毒症的脓宫致脓毒症(P-脓毒症-).根据重要的临床评分和总白细胞计数，将患有子宫积脓的母猪（n=52）分为P型败血症（n=28）和P型败血症（n=24）。一组非积脓母犬（n=12）作为对照。IL6，IL8，TNFα转录本的相对倍数变化，IL10、PTGS2、mPGES1和PGFS，SLPI,通过定量聚合酶链反应测定S100A8，S100A12和eNOS。此外，ELISA法检测血清IL6、IL8、IL10、SLPI和前列腺素F2α代谢物(PGFM)的浓度。P型脓毒症组S100A12和SLPI的相对倍数变化以及IL6和SLPI的平均浓度显着（p<0.05）高于P型脓毒症组。受试者工作特征分析显示，血清IL6的诊断灵敏度为78.6％，阳性似然比（LR）为2.09，临界值为15.7pg/mL，可诊断P-脓毒症病例。同样，血清SLPI的敏感性为84.6%，LR+为2.23，临界值为2.0pg/mL。结论是，SLPI和IL6将作为母犬子宫积脓导致的败血症的推定生物标志物。监测SLPI和IL6将是既定的血液生化参数的有用辅助手段，可以定制治疗策略并决定管理患有危重病的子宫积母犬。
