Abstract：
UNASSIGNED: Chromosomal abnormalities play an important role in diagnosis and prognosis of hematological diseases.
UNASSIGNED: The aim of the present study was to study the pattern and frequency of chromosomal aberrations in acute myeloid leukemia (AML) subgroups from western India.
UNASSIGNED: A retrospective study was conducted through evaluating laboratory proforma which were filled during 2005 to 2014 for diagnosis and treatment of AML subjects.
UNASSIGNED: We have studied chromosomal aberrations in 282 subjects with AML from western India. AML patients were sub-grouped according to FAB classification. Cytogenetic study using conventional cytogenetics (GTG-banding) and Fluorescence in situ hybridization (FISH) was carried out using FISH probes (AML1/ETO, PML/RARA, CBFB).
UNASSIGNED: Student\'s t test for continuous variables and Pearson\'s Chi-squared test for categorical variables were used to identify the relationship between variables.
UNASSIGNED: Cytomorphological study revealed AML- M3 as most frequent (32.3%) group followed by AML-M2 (25.2%) and AML-M4 (19.9%). Chromosomal abnormalities were identified in 145 (51.42%) of the total AML cases. A high frequency (38.6%) of chromosomal abnormalities was identified in AML-M3 subgroup as compared to AML-M2 (31%) and AML-M4 (20.6%).
UNASSIGNED: Cytogenetic study is important for the diagnosis and management of the AML patients. Our study identified chromosomal abnormalities in AML subgroups with varied frequencies. It is important in diagnosis and monitoring of the disease. As younger AML patients were more affected in our study, etiological factors such as environmental factors need to be studied. Combination of conventional cytogenetics and FISH has an advantage of identifying high frequency of chromosomal aberrations in AML patients.
UNASSIGNED: The aim of the present study was to study the pattern and frequency of chromosomal aberrations in acute myeloid leukemia (AML) subgroups from western India.
UNASSIGNED: A retrospective study was conducted through evaluating laboratory proforma which were filled during 2005 to 2014 for diagnosis and treatment of AML subjects.
UNASSIGNED: We have studied chromosomal aberrations in 282 subjects with AML from western India. AML patients were sub-grouped according to FAB classification. Cytogenetic study using conventional cytogenetics (GTG-banding) and Fluorescence in situ hybridization (FISH) was carried out using FISH probes (AML1/ETO, PML/RARA, CBFB).
UNASSIGNED: Student\'s t test for continuous variables and Pearson\'s Chi-squared test for categorical variables were used to identify the relationship between variables.
UNASSIGNED: Cytomorphological study revealed AML- M3 as most frequent (32.3%) group followed by AML-M2 (25.2%) and AML-M4 (19.9%). Chromosomal abnormalities were identified in 145 (51.42%) of the total AML cases. A high frequency (38.6%) of chromosomal abnormalities was identified in AML-M3 subgroup as compared to AML-M2 (31%) and AML-M4 (20.6%).
UNASSIGNED: Cytogenetic study is important for the diagnosis and management of the AML patients. Our study identified chromosomal abnormalities in AML subgroups with varied frequencies. It is important in diagnosis and monitoring of the disease. As younger AML patients were more affected in our study, etiological factors such as environmental factors need to be studied. Combination of conventional cytogenetics and FISH has an advantage of identifying high frequency of chromosomal aberrations in AML patients.
摘要：
■染色体异常在血液病的诊断和预后中起重要作用。
■本研究的目的是研究印度西部急性髓性白血病（AML）亚组染色体畸变的模式和频率。
■通过评估2005年至2014年期间用于诊断和治疗AML受试者的实验室形式进行了回顾性研究。
■我们研究了来自印度西部的282名AML患者的染色体畸变。AML患者根据FAB分类进行分组。使用FISH探针（AML1/ETO，PML/RARA,CBFB)。
■连续变量的学生t检验和分类变量的皮尔逊卡方检验用于确定变量之间的关系。
■细胞形态学研究显示，AML-M3是最常见的组（32.3％），其次是AML-M2（25.2％）和AML-M4（19.9％）。在145例（51.42％）的AML病例中发现了染色体异常。与AML-M2（31％）和AML-M4（20.6％）相比，在AML-M3亚组中发现了高频率（38.6％）的染色体异常。
■细胞遗传学研究对于AML患者的诊断和治疗很重要。我们的研究确定了AML亚群中染色体异常的频率不同。它在疾病的诊断和监测中很重要。由于年轻的AML患者在我们的研究中受到的影响更大，环境因素等病因学因素需要研究。常规细胞遗传学和FISH的组合具有在AML患者中识别高频率的染色体畸变的优势。
■本研究的目的是研究印度西部急性髓性白血病（AML）亚组染色体畸变的模式和频率。
■通过评估2005年至2014年期间用于诊断和治疗AML受试者的实验室形式进行了回顾性研究。
■我们研究了来自印度西部的282名AML患者的染色体畸变。AML患者根据FAB分类进行分组。使用FISH探针（AML1/ETO，PML/RARA,CBFB)。
■连续变量的学生t检验和分类变量的皮尔逊卡方检验用于确定变量之间的关系。
■细胞形态学研究显示，AML-M3是最常见的组（32.3％），其次是AML-M2（25.2％）和AML-M4（19.9％）。在145例（51.42％）的AML病例中发现了染色体异常。与AML-M2（31％）和AML-M4（20.6％）相比，在AML-M3亚组中发现了高频率（38.6％）的染色体异常。
■细胞遗传学研究对于AML患者的诊断和治疗很重要。我们的研究确定了AML亚群中染色体异常的频率不同。它在疾病的诊断和监测中很重要。由于年轻的AML患者在我们的研究中受到的影响更大，环境因素等病因学因素需要研究。常规细胞遗传学和FISH的组合具有在AML患者中识别高频率的染色体畸变的优势。
