Abstract：
Skeletal muscle can adjust to changes in physiological and pathological environments by regenerating using myogenic progenitor cells or adapting muscle fiber sizes and types, metabolism, and contraction ability. To study these changes, muscle samples should be appropriately prepared. Therefore, reliable techniques to accurately analyze and evaluate skeletal muscle phenotypes are required. However, although technical approaches to genetically investigating skeletal muscle are improving, the fundamental strategies for capturing muscle pathology are the same over the decades. Hematoxylin and eosin (H&E) staining or antibodies are the simplest and standard methodologies for assessing skeletal muscle phenotypes. In this chapter, we describe fundamental techniques and protocols for inducing skeletal muscle regeneration by using chemicals and cell transplantation, in addition to methods of preparing and evaluating skeletal muscle samples.
摘要：
骨骼肌可以通过使用生肌祖细胞再生或适应肌纤维大小和类型来适应生理和病理环境的变化，新陈代谢，和收缩能力。为了研究这些变化，应适当准备肌肉样本。因此,需要可靠的技术来准确分析和评估骨骼肌表型。然而,尽管对骨骼肌进行基因研究的技术方法正在改进，几十年来，捕获肌肉病理的基本策略是相同的。苏木精和伊红(H&E)染色或抗体是评估骨骼肌表型的最简单和标准的方法。在这一章中,我们描述了通过使用化学物质和细胞移植诱导骨骼肌再生的基本技术和方案，除了制备和评估骨骼肌样品的方法。
