Abstract：
Beginning in July 2019, numerous free-ranging brown anoles (Anolis sagrei), an invasive lizard species in Florida, USA, were reported with large, soft, subcutaneous masses and disfiguring facial swellings. Postmortem evaluations of six affected animals, including cytology, histology, and electron microscopy, identified the presence of myriad chain-forming coccoid bacteria surrounded by a prominent clear capsule and abundant lightly basophilic matrix material with minimal associated granulomatous inflammation and effacement of normal tissue. Standard PCR and sequencing of the lesions revealed 100% nucleotide identity to Enterococcus lacertideformus. This bacterium was first observed in 2014 as the cause of a severe, multisystemic infection in several species of lizards (geckos and skinks) on Christmas Island, an Australian external territory in the Indian Ocean. Previously, analysis of E. lacertideformus had been hindered by an inability to grow the bacterium in standard culture conditions. We successfully cultured the organism on primary anole kidney cells. Given the growing recognition of host species diversity and geographic distribution noted for this organism, there is potential concern for spread to native North American lizards, especially the green anole (Anolis carolinensis), whose population numbers have apparently decreased due to introduced brown anoles.
摘要：
从2019年7月开始，许多自由放养的棕色anoles（Anolissagrei），佛罗里达的一种入侵蜥蜴物种，美国,据报道，软,皮下肿块和面部肿胀。六只受影响动物的死后评估，包括细胞学,组织学,和电子显微镜，确定存在无数形成链的球形细菌，这些细菌被突出的透明胶囊和丰富的轻度嗜碱性基质材料包围，并具有最小的相关肉芽肿性炎症和正常组织的消退。标准PCR和病变测序显示100％的核苷酸相同性肠球菌。这种细菌在2014年首次被观察到是严重的，圣诞岛上几种蜥蜴（壁虎和龙骨）的多系统感染，澳大利亚在印度洋的外部领土。以前,由于无法在标准培养条件下生长细菌，因此阻碍了对泪菌的分析。我们成功地在原代肾细胞上培养了该生物。鉴于对这种生物的寄主物种多样性和地理分布的认识日益提高，有可能会传播到北美本土蜥蜴，尤其是绿色的Anole（Anoliscarolinensis），由于引入了棕色的乙醇，其种群数量明显减少。
