Abstract：
Cleome viscosa L., a member of the family Cleomaceae, is a potential medicinal plant, known for several bioactive properties such as: anticancer, antidiabetic, antioxidant, anti-inflammatory, antimicrobial, wound healing, etc. Our study aimed to isolate a bioactive compound and assess its antibacterial activity. The crystal compound imperatorin was isolated and reported for the first time from the aerial parts of C. viscosa. The isolation was made using silica gel (100-200 mesh) column chromatography. The structure of imperatorin was investigated through single-crystal XRD, unit cell molecules, FTIR, and ESI-MS spectral analysis. The results validated imperatorin\'s triclinic crystal structure and P2i/c distance group. The electronic structure was also calculated (4.28/6.21 D) along with the frontier molecular orbital, dipole moment, atomic charges, and electrostatic map of particles in gaseous stage and active site. Imperatorin showed highest activity at 40 µg/mL concentration against Gram + ve bacteria: Staphylococcus aureus (3 ± 0.2 mm), Bacillus subtilis (3 ± 0.6 mm), and Gram -ve bacteria: Klebsiella pneumoniae (3 ± 0.2 mm), Escherichia coli (5 ± 0.2 mm). The study highlights that the compound can be isolated in larger quantities as the plant is easily available across the tropics.
摘要：
Cleome粘胶L.，清理科的一员,是一种潜在的药用植物，以几种生物活性特性而闻名，例如：抗癌，抗糖尿病药,抗氧化剂，抗炎,抗菌，伤口愈合，等。我们的研究旨在分离生物活性化合物并评估其抗菌活性。晶体化合物欧前胡素是从粘藻的地上部分分离并首次报道。使用硅胶(100-200目)柱色谱法进行分离。通过单晶XRD研究了欧前胡素的结构，单位细胞分子，FTIR,和ESI-MS光谱分析。结果验证了欧前胡素的三斜晶系晶体结构和P2i/c距离组。还计算了电子结构（4.28/6.21D）以及前沿分子轨道，偶极矩,原子电荷,气体阶段和活性部位的颗粒静电图。Imperatorin在40µg/mL浓度下对革兰氏细菌：金黄色葡萄球菌（3±0.2mm）的活性最高，枯草芽孢杆菌(3±0.6mm),和革兰氏-ve细菌：肺炎克雷伯菌（3±0.2mm），大肠杆菌(5±0.2mm)。该研究强调，由于该植物在热带地区很容易获得，因此可以大量分离该化合物。
