Abstract：
Recently, selenium nanoparticles have been drawing attention worldwide, and it is crucial to increase the stability of nano-Se. Morinda officinalis polysaccharides (MOP) are the main active component in Morinda officinalis radix. However, their low activity has limited their application. A novel selenium nanoparticle (Se-MOP) was prepared to solve these problems using MOP as a dispersant. The zeta potential was measured to evaluate the stability, and UV and ATR-FTIR were used to investigate the binding type of selenium and MOP. The morphology was observed by the TEM method. Furthermore, the inhibitory effect on five selected cancer cells (HepG2, MCF-7, AGS, PC9, and HCT8) was evaluated, showing remarkable inhibition of all five cancer cells. The mechanism of inhibition was also investigated by cell circle assay, and it was found that Se-MOP could induce cell circle G0/G1 phase arrest. Immune-enhancing activities were evaluated by measuring the proliferation and cytokines of mouse spleen lymphocytes in vitro and quantitative RT-PCR. The results indicated that single stimulation of Se-MOP and synergistic stimulation with PHA or LPS increased immune capacity and improved immune by increasing the expression of cytokines.
摘要：
最近,硒纳米颗粒已经引起了全世界的关注，提高纳米硒的稳定性至关重要。巴林达多糖(MOP)是巴林达的主要活性成分。然而,它们的低活性限制了它们的应用。为了解决这些问题，使用MOP作为分散剂制备了一种新型的硒纳米颗粒（Se-MOP）。测量ζ电位以评估稳定性，采用UV和ATR-FTIR研究了硒与MOP的结合类型。通过TEM方法观察形态。此外，对五种选定的癌细胞(HepG2，MCF-7，AGS，PC9和HCT8)进行了评估，对所有五种癌细胞都有显著的抑制作用。还通过细胞周期测定研究了抑制的机制，发现Se-MOP可以诱导细胞周期G0/G1期阻滞。通过体外测定小鼠脾淋巴细胞的增殖和细胞因子并进行定量RT-PCR来评估免疫增强活性。结果表明，Se-MOP的单次刺激和PHA或LPS的协同刺激可通过增加细胞因子的表达来增加免疫能力并改善免疫力。
