Abstract：
BACKGROUND: Autoimmune hepatitis (AIH) is associated with periportal infiltration by plasma cells. Plasma cell detection is routinely performed through hematoxylin and eosin (H&E) staining. The present study aimed to assess the utility of CD138, an immunohistochemical plasma cell marker, in the evaluation of AIH.
METHODS: A retrospective study was conducted, in which cases consistent with AIH, within the time frame of 2001 and 2011, were collected. Routine H&E-stained sections were used for evaluation. CD138 immunohistochemistry (IHC) was performed to detect plasma cells.
RESULTS: Sixty biopsies were included. In the H&E group, the median and interquartile range (IQR) was 6 (4-9) plasma cells/high power field (HPF) and was 10 (IQR 6-20) plasma cells/HPF in the CD138 group (p < 0.001). There was a significant correlation between the number of plasma cells determined by H&E and CD138 (p = 0.31, p = 0.01). No significant correlation was found between the number of plasma cells determined by CD138 and IgG level (p = 0.21, p = 0.09) or stage of fibrosis (p = 0.12, p = 0.35), or between IgG level and stage of fibrosis (p = 0.17, p = 0.17). No significant correlation was found between the treatment response and the number of plasma cells determined by H&E (p = 0.11, p = 0.38), CD138 (p = 0.07, p = 0.55), or stage of fibrosis (p = 0.16, p = 0.20). CD138 expression was different between the treatment response groups (p = 0.04).
CONCLUSIONS: CD138 increased the detection of plasma cells in liver biopsies of patients with AIH, when compared with routine H&E staining. However, there was no correlation between the number of plasma cells determined by CD138 and serum IgG levels, stage of fibrosis, or response to treatment.
METHODS: A retrospective study was conducted, in which cases consistent with AIH, within the time frame of 2001 and 2011, were collected. Routine H&E-stained sections were used for evaluation. CD138 immunohistochemistry (IHC) was performed to detect plasma cells.
RESULTS: Sixty biopsies were included. In the H&E group, the median and interquartile range (IQR) was 6 (4-9) plasma cells/high power field (HPF) and was 10 (IQR 6-20) plasma cells/HPF in the CD138 group (p < 0.001). There was a significant correlation between the number of plasma cells determined by H&E and CD138 (p = 0.31, p = 0.01). No significant correlation was found between the number of plasma cells determined by CD138 and IgG level (p = 0.21, p = 0.09) or stage of fibrosis (p = 0.12, p = 0.35), or between IgG level and stage of fibrosis (p = 0.17, p = 0.17). No significant correlation was found between the treatment response and the number of plasma cells determined by H&E (p = 0.11, p = 0.38), CD138 (p = 0.07, p = 0.55), or stage of fibrosis (p = 0.16, p = 0.20). CD138 expression was different between the treatment response groups (p = 0.04).
CONCLUSIONS: CD138 increased the detection of plasma cells in liver biopsies of patients with AIH, when compared with routine H&E staining. However, there was no correlation between the number of plasma cells determined by CD138 and serum IgG levels, stage of fibrosis, or response to treatment.
摘要：
背景：自身免疫性肝炎（AIH）与浆细胞的门静脉浸润有关。通过苏木精和伊红(H&E)染色常规进行浆细胞检测。本研究旨在评估免疫组织化学浆细胞标志物CD138的实用性，在AIH的评估中。
方法：进行了回顾性研究，在与AIH一致的情况下，在2001年和2011年的时间范围内，收集。常规H&E染色的切片用于评估。进行CD138免疫组织化学(IHC)以检测浆细胞。
结果：包括60例活检。在H&E组,CD138组的中位数和四分位数间距(IQR)为6(4~9)个浆细胞/高倍视野(HPF)和10(IQR6~20)个浆细胞/HPF(p<0.001).通过H&E和CD138测定的浆细胞数量之间存在显著相关性(p=0.31,p=0.01)。CD138与IgG水平（p=0.21，p=0.09）或纤维化阶段（p=0.12，p=0.35）之间没有发现显着相关性，或IgG水平和纤维化阶段之间（p=0.17，p=0.17）。治疗反应与H&E测定的浆细胞数之间无显著相关性(p=0.11,p=0.38),CD138（p=0.07，p=0.55），或纤维化阶段（p=0.16，p=0.20）。CD138表达在治疗响应组之间是不同的(p=0.04)。
结论：CD138增加了AIH患者肝活检中浆细胞的检测，与常规H&E染色相比。然而,CD138测定的浆细胞数量与血清IgG水平之间没有相关性，纤维化阶段，或对治疗的反应。
方法：进行了回顾性研究，在与AIH一致的情况下，在2001年和2011年的时间范围内，收集。常规H&E染色的切片用于评估。进行CD138免疫组织化学(IHC)以检测浆细胞。
结果：包括60例活检。在H&E组,CD138组的中位数和四分位数间距(IQR)为6(4~9)个浆细胞/高倍视野(HPF)和10(IQR6~20)个浆细胞/HPF(p<0.001).通过H&E和CD138测定的浆细胞数量之间存在显著相关性(p=0.31,p=0.01)。CD138与IgG水平（p=0.21，p=0.09）或纤维化阶段（p=0.12，p=0.35）之间没有发现显着相关性，或IgG水平和纤维化阶段之间（p=0.17，p=0.17）。治疗反应与H&E测定的浆细胞数之间无显著相关性(p=0.11,p=0.38),CD138（p=0.07，p=0.55），或纤维化阶段（p=0.16，p=0.20）。CD138表达在治疗响应组之间是不同的(p=0.04)。
结论：CD138增加了AIH患者肝活检中浆细胞的检测，与常规H&E染色相比。然而,CD138测定的浆细胞数量与血清IgG水平之间没有相关性，纤维化阶段，或对治疗的反应。
