PDF(Pubmed)
Abstract：
Seaweed extracts are a prominent class of biostimulants that enhance plant health and tolerance to biotic and abiotic stresses due to their unique bioactive components. However, the mechanisms of action of biostimulants are still unknown. Here, we have used a metabolomic approach, a UHPLC-MS method, to uncover the mechanisms induced following application to Arabidopsis thaliana of a seaweed extract derived from Durvillaea potatorum and Ascophyllum nodosum. We have identified, following the application of the extract, key metabolites and systemic responses in roots and leaves across 3 timepoints (0, 3, 5 days). Significant alterations in metabolite accumulation or reduction were found for those belonging to broad groups of compounds such as lipids, amino acids, and phytohormones; and secondary metabolites such as phenylpropanoids, glucosinolates, and organic acids. Strong accumulations of TCA cycle and N-containing and defensive metabolites such as glucosinolates were also found revealing the enhancement of carbon and nitrogen metabolism and defence systems. Our study has demonstrated that application of seaweed extract dramatically altered the metabolomic profiles of Arabidopsis and revealed differences in roots and leaves that varied across the timepoints tested. We also show clear evidence of systemic responses that were initiated in the roots and resulted in metabolic alterations in the leaves. Collectively, our results suggest that this seaweed extract promotes plant growth and activates defence systems by altering various physiological processes at the individual metabolite level.
摘要：
海藻提取物是一类突出的生物刺激剂,其由于其独特的生物活性成分而增强植物健康和对生物和非生物胁迫的耐受性。然而,生物刺激剂的作用机制仍然未知。这里,我们使用了代谢组学方法，UHPLC-MS方法，揭示将来自Durvillaeapotatorum和Ascophyllumnodosum的海藻提取物应用于拟南芥后诱导的机制。我们已经确定,在提取物的应用之后，在3个时间点（0、3、5天），根和叶中的关键代谢产物和系统反应。代谢物积累或减少的显着变化被发现属于广泛的化合物，如脂质，氨基酸,和植物激素；和次级代谢产物，如苯丙素，芥子油苷,有机酸。还发现了TCA循环和含N和防御性代谢物如芥子油苷的强烈积累，揭示了碳和氮代谢和防御系统的增强。我们的研究表明，海藻提取物的应用极大地改变了拟南芥的代谢组学特征，并揭示了根和叶在测试时间点之间的差异。我们还显示了在根部开始并导致叶片代谢改变的系统性反应的明确证据。总的来说，我们的结果表明，这种海藻提取物通过改变个体代谢物水平的各种生理过程来促进植物生长并激活防御系统。
