PDF(Pubmed)
Abstract：
The aim of this study was to design and optimize a cold atmospheric plasma (CAP) device that could be applied in an oral environment and to study its effects on plaque biofilm metabolism and regrowth, as well as microbial flora composition and enamel demineralization.
CAP was obtained through a dielectric barrier discharge device; the optical properties were analyzed using emission spectroscopy. The electrochemical analysis of plasma devices includes voltametric characteristic curves and Lissajous. The Streptococcus mutans (UA159) and saliva biofilms were treated in vitro, and the effects of CAP on biofilm metabolism were investigated using MTT and lactate dehydrogenase assays. The duration of antibacterial activity on biofilms was examined, scanning electron microscopy was used to observe the morphology of biofilms, and 16S rRNA sequencing was used to explore the influence of CAP on the microbial flora composition of saliva biofilms. An in vitro model of biofilm-enamel demineralization was designed, and the effect of CAP on enamel demineralization was evaluated by micro surface hardness and micro-CT analysis.
CAP had antibacterial proliferative ability toward Streptococcus mutans biofilms and saliva biofilms and was stronger than ultraviolet under the same tested conditions. After 24 h, the antibacterial effect disappeared, which proved the short-term timeliness of its bactericidal ability. CAP can inhibit the acid production of biofilms, and its inhibitory effect on saliva biofilms can be extended to 24 h. CAP had a strong ability to regulate the composition of plaque biofilms, especially for Lactococcus proliferation, a major acid-producing bacterium in microcosm biofilms. The CAP-treated enamels were more acid-tolerant than non-treated controls.
CAP had an explicit bactericidal effect on caries-related biofilms, which is a short-term antibacterial effect. It can inhibit the acid production of biofilms and has a downregulation effect on Lactococcus in saliva biofilms. CAP can help reduce demineralization of enamel.
CAP was obtained through a dielectric barrier discharge device; the optical properties were analyzed using emission spectroscopy. The electrochemical analysis of plasma devices includes voltametric characteristic curves and Lissajous. The Streptococcus mutans (UA159) and saliva biofilms were treated in vitro, and the effects of CAP on biofilm metabolism were investigated using MTT and lactate dehydrogenase assays. The duration of antibacterial activity on biofilms was examined, scanning electron microscopy was used to observe the morphology of biofilms, and 16S rRNA sequencing was used to explore the influence of CAP on the microbial flora composition of saliva biofilms. An in vitro model of biofilm-enamel demineralization was designed, and the effect of CAP on enamel demineralization was evaluated by micro surface hardness and micro-CT analysis.
CAP had antibacterial proliferative ability toward Streptococcus mutans biofilms and saliva biofilms and was stronger than ultraviolet under the same tested conditions. After 24 h, the antibacterial effect disappeared, which proved the short-term timeliness of its bactericidal ability. CAP can inhibit the acid production of biofilms, and its inhibitory effect on saliva biofilms can be extended to 24 h. CAP had a strong ability to regulate the composition of plaque biofilms, especially for Lactococcus proliferation, a major acid-producing bacterium in microcosm biofilms. The CAP-treated enamels were more acid-tolerant than non-treated controls.
CAP had an explicit bactericidal effect on caries-related biofilms, which is a short-term antibacterial effect. It can inhibit the acid production of biofilms and has a downregulation effect on Lactococcus in saliva biofilms. CAP can help reduce demineralization of enamel.
摘要：
■这项研究的目的是设计和优化可应用于口腔环境的冷大气等离子体（CAP）装置，并研究其对牙菌斑生物膜代谢和再生长的影响，以及微生物菌群组成和牙釉质脱矿。
■CAP是通过介质阻挡放电装置获得的；使用发射光谱法分析了光学性质。等离子体器件的电化学剖析包含伏安特征曲线和Lissajous。变形链球菌（UA159）和唾液生物膜在体外处理，采用MTT法和乳酸脱氢酶法研究了CAP对生物膜代谢的影响。检查了生物膜上抗菌活性的持续时间，用扫描电镜观察生物膜的形态,采用16SrRNA测序技术探讨CAP对唾液生物膜微生物菌群组成的影响。设计了生物膜-釉质脱矿的体外模型,通过显微表面硬度和显微CT分析评价CAP对牙釉质脱矿的影响。
■CAP对变形链球菌生物膜和唾液生物膜具有抗菌增殖能力，并且在相同的测试条件下比紫外线更强。24小时后，抗菌效果消失了，证明了其杀菌能力的短期时效性。CAP可以抑制生物膜的产酸，对唾液生物膜的抑制作用可延长至24h。CAP对菌斑生物膜的组成有很强的调节作用,特别是对于乳球菌的增殖，微观世界生物膜中的主要产酸细菌。CAP处理的搪瓷比未处理的对照更耐酸。
■CAP对龋齿相关生物膜有明显的杀菌作用，这是一种短期的抗菌作用。它可以抑制生物膜的产酸，并对唾液生物膜中的乳球菌具有下调作用。CAP可以帮助减少牙釉质的脱矿质。
■CAP是通过介质阻挡放电装置获得的；使用发射光谱法分析了光学性质。等离子体器件的电化学剖析包含伏安特征曲线和Lissajous。变形链球菌（UA159）和唾液生物膜在体外处理，采用MTT法和乳酸脱氢酶法研究了CAP对生物膜代谢的影响。检查了生物膜上抗菌活性的持续时间，用扫描电镜观察生物膜的形态,采用16SrRNA测序技术探讨CAP对唾液生物膜微生物菌群组成的影响。设计了生物膜-釉质脱矿的体外模型,通过显微表面硬度和显微CT分析评价CAP对牙釉质脱矿的影响。
■CAP对变形链球菌生物膜和唾液生物膜具有抗菌增殖能力，并且在相同的测试条件下比紫外线更强。24小时后，抗菌效果消失了，证明了其杀菌能力的短期时效性。CAP可以抑制生物膜的产酸，对唾液生物膜的抑制作用可延长至24h。CAP对菌斑生物膜的组成有很强的调节作用,特别是对于乳球菌的增殖，微观世界生物膜中的主要产酸细菌。CAP处理的搪瓷比未处理的对照更耐酸。
■CAP对龋齿相关生物膜有明显的杀菌作用，这是一种短期的抗菌作用。它可以抑制生物膜的产酸，并对唾液生物膜中的乳球菌具有下调作用。CAP可以帮助减少牙釉质的脱矿质。
