Abstract：
Accurately quantifying trophic positions (TP) to describe food web structure is an important element in studying pollutant bioaccumulation. In recent years, compound-specific nitrogen isotopic analysis of amino acids (AAs-N-CSIA) has been progressively applied as a potentially reliable tool for quantifying TP, facilitating a better understanding of pollutant food web transfer. Therefore, this review provides an overview of the analytical procedures, applications, and limitations of AAs-N-CSIA in pollutant (halogenated organic pollutants (HOPs) and heavy metals) bioaccumulation studies. We first summarize studies on the analytical techniques of AAs-N-CSIA, including derivatization, instrumental analysis, and data processing methods. The N-pivaloyl-i-propyl-amino acid ester method is a more suitable AAs derivatization method for quantifying TP. The AAs-N-CSIA application in pollutant bioaccumulation studies (e.g., Hg, MeHg, and HOPs) is discussed, and its application in conjunction with various techniques (e.g., spatial analysis, food source analysis, and compound tracking techniques, etc.) to research the influence of pollutant levels on organisms is summarized. Finally, the limitations of AAs-N-CSIA in pollutant bioaccumulation studies are discussed, including the use of single empirical values of βglu/phe and TDFglu/phe that result in large errors in TP quantification. The weighted βglu/phe and the multi-TDFglu/phe models are still challenging to solve for accurate TP quantification of omnivores; however, factors affecting the variation of βglu/phe and TDFglu/phe are unclear, especially the effect of pollutant bioaccumulation in organisms on internal AA metabolic processes.
摘要：
准确量化营养位置（TP）以描述食物网结构是研究污染物生物累积的重要因素。近年来,氨基酸的化合物特异性氮同位素分析（AAs-N-CSIA）已逐步用作定量TP的潜在可靠工具，有助于更好地了解污染物食物网的转移。因此,这篇综述概述了分析程序，应用程序,以及AAs-N-CSIA在污染物（卤化有机污染物（HOP）和重金属）生物累积研究中的局限性。我们首先总结了AAs-N-CSIA分析技术的研究，包括衍生化，仪器分析,和数据处理方法。N-新戊酰基-异丙基-氨基酸酯方法是用于定量TP的更合适的AAs衍生化方法。AAs-N-CSIA在污染物生物累积研究中的应用(例如，Hg,MeHg,和HOPS)进行了讨论，及其与各种技术的结合应用(例如，空间分析,食物来源分析,和复合跟踪技术，等。)总结了污染物水平对生物体的影响。最后,讨论了AAs-N-CSIA在污染物生物累积研究中的局限性，包括使用βglu/phe和TDFglu/phe的单个经验值，导致TP定量存在较大误差。加权βglu/phe和多TDFglu/phe模型仍然难以解决杂食动物的准确TP量化问题；然而，影响βglu/phe和TDFglu/phe变异的因素尚不清楚，特别是污染物在生物体内的生物累积对AA内部代谢过程的影响。
