关键词: LAMP PCR Plant disease diagnosis early detection in-field portable devices plant pathogens

来  源:   DOI:10.1002/ps.7471

Abstract:
Next-generation methods for early disease detection in crops. A review. (https://doi.org/10.1002/ps.7471) Daniela Trippa, Riccardo Scalenghe, Marcos Fernando Basso, Stefano Panno, Salvatore Davino, Chiara Morone, Antonio Giovino, Safa Oufensou, Nicola Luchi, Sanaz Yousefi and Federico Martinelli. The above article from Pest Management Science, published online on 24 March 2023 in Wiley Online Library (http://wileyonlinelibrary.com) has been withdrawn by agreement among the Journal Editors-in-Chief, Dr Stephen Duke and Professor Yidong Wu, and John Wiley & Sons Ltd on behalf of the Society of Chemical Industry. The withdrawal has been agreed because of extensive revisions after acceptance of the paper. The new study is being resubmitted to the journal for formal review. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
摘要:
背景:植物病原体在本领域通常通过它们可能引起的典型疾病症状来鉴定。有效的早期检测和识别病原体是采取有效的管理措施以减少或防止其传播以减轻疾病的负面影响的基本程序。在这里,在这篇综述中提出并讨论了早期检测病原体的传统和创新方法。此外,他们强调了当前技术的主要优点和局限性。
结果:用于植物病原体鉴定的传统诊断技术通常集中在DNA上,RNA(当分子方法),和蛋白质或肽(当血清学方法)的病原体。基于主要酶联免疫吸附测定(ELISA)的血清学方法是用于病原体检测的最常用方法,因为它们具有高通量潜力和低成本。这种技术对于无症状感染阶段的许多病原体检测不是特别可靠和足够灵敏。对于实验室中不可培养的病原体,基于核酸的技术是一致的病原体检测或鉴定的最佳选择。横向流动系统是创新的工具,即使在现场条件下也能快速准确地获得结果,但是他们有需要克服的敏感性问题。在上一代便携式热循环仪上进行的PCR测定可以在原位提供快速检测结果。
结论:便携式仪器的出现可以加快病原体检测,降低商业成本,并有可能彻底改变植物病理学。这篇综述提供了有关有效检测不同植物病原体的当前方法和程序的信息。本文受版权保护。保留所有权利。
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