Abstract：
The investigation of the lipid-protein microdomains of the plasmalemma isolated with the aid of the non-detergent technique in the zones of the sucrose density gradient after high-speed centrifugation from the tissue pieces of beet roots, which underwent oxidative stress, was conducted. The microdomains, whose lipid composition - according to the definition - allowed us to classify them as rafts, were studied. After the exposure to oxidative stress (100 mM hydrogen peroxide), the variations in the composition of membrane lipids bound up mainly with the elevations of the content of raft-forming lipids (sterols, sterol esters). Oxidative stress provoked redistribution in the composition of sterols, which led to an elevation in the content of campesterol and in the ratio of stigmasterol/sitosterol. Furthermore, the variations were registered in the content of phospholipids and phosphoglycerolipids, which are capable of stabilizing the lamellar structure of membranes. The results obtained allow one to assume that under the oxidative stress, variations in the composition of lipids in microdomains of the plasma membrane can take place. These variations may influence the functioning of the membranes, and the membranes may participate in the protection of the plant cell.
摘要：
从甜菜根的组织碎片高速离心后，借助非去污剂技术在蔗糖密度梯度区域中分离出的质膜的脂质-蛋白质微区的研究，经历了氧化应激，进行了。微域，他们的脂质成分-根据定义-允许我们将它们分类为筏，被研究过。暴露于氧化应激（100mM过氧化氢）后，膜脂质组成的变化主要与筏形成脂质（固醇，甾醇酯)。氧化应激在甾醇的组成中引起了重新分布，这导致菜油甾醇的含量和豆甾醇/谷甾醇的比例升高。此外，这些变化记录在磷脂和磷酸甘油酯的含量上，能够稳定膜的层状结构。获得的结果允许人们假设在氧化应激下，质膜微域中脂质组成的变化可能发生。这些变化可能会影响膜的功能，膜可能参与植物细胞的保护。
