PDF(Pubmed)
Abstract：
UNASSIGNED: The aim of this study was to explore the histopathological and genetic changes in the submandibular glands after duct ligation and provide important clues to functional regeneration.
UNASSIGNED: We established a rat salivary gland duct ligation model and observed pathological changes in the rat submandibular gland on day 1 and weeks 1, 2, 3, and 4 using hematoxylin and eosin staining, Alcian blue-periodic acid Schiff staining, Masson staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), and immunohistochemical staining. RNA sequencing was performed on normal salivary glands and those from the ligation model after 1 week. Significantly differentially expressed genes were selected, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed.
UNASSIGNED: Apoptosis levels and histological and functional KEGG pathway analyses showed that injury to the salivary gland after ligation gradually increased. The TGF-β pathway was activated and promoted fibrosis. RNA sequencing results and further verification of samples at week 1 showed that the NF-κB pathway plays a vital role in salivary gland atrophy.
UNASSIGNED: Our results detailed the pathological changes in the submandibular gland after ligation and the important functions of the NF-κB pathway.
UNASSIGNED: We established a rat salivary gland duct ligation model and observed pathological changes in the rat submandibular gland on day 1 and weeks 1, 2, 3, and 4 using hematoxylin and eosin staining, Alcian blue-periodic acid Schiff staining, Masson staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), and immunohistochemical staining. RNA sequencing was performed on normal salivary glands and those from the ligation model after 1 week. Significantly differentially expressed genes were selected, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed.
UNASSIGNED: Apoptosis levels and histological and functional KEGG pathway analyses showed that injury to the salivary gland after ligation gradually increased. The TGF-β pathway was activated and promoted fibrosis. RNA sequencing results and further verification of samples at week 1 showed that the NF-κB pathway plays a vital role in salivary gland atrophy.
UNASSIGNED: Our results detailed the pathological changes in the submandibular gland after ligation and the important functions of the NF-κB pathway.
摘要：
■这项研究的目的是探索导管结扎后下颌下腺的组织病理学和遗传变化，并为功能再生提供重要线索。
■我们建立了大鼠唾液腺导管结扎模型，并在第1天和第1、2、3和4周采用苏木精和伊红染色观察了大鼠下颌下腺的病理变化，阿尔辛蓝高碘酸希夫染色，Masson染色,末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL），和免疫组织化学染色。在1周后对正常唾液腺和来自连接模型的唾液腺进行RNA测序。选择显著差异表达的基因，进行了基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径分析。
■细胞凋亡水平和组织学和功能KEGG通路分析表明，结扎后唾液腺的损伤逐渐增加。TGF-β途径被激活并促进纤维化。RNA测序结果和第1周样本的进一步验证表明，NF-κB通路在唾液腺萎缩中起着至关重要的作用。
■我们的结果详细介绍了结扎后颌下腺的病理变化以及NF-κB通路的重要功能。
■我们建立了大鼠唾液腺导管结扎模型，并在第1天和第1、2、3和4周采用苏木精和伊红染色观察了大鼠下颌下腺的病理变化，阿尔辛蓝高碘酸希夫染色，Masson染色,末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL），和免疫组织化学染色。在1周后对正常唾液腺和来自连接模型的唾液腺进行RNA测序。选择显著差异表达的基因，进行了基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径分析。
■细胞凋亡水平和组织学和功能KEGG通路分析表明，结扎后唾液腺的损伤逐渐增加。TGF-β途径被激活并促进纤维化。RNA测序结果和第1周样本的进一步验证表明，NF-κB通路在唾液腺萎缩中起着至关重要的作用。
■我们的结果详细介绍了结扎后颌下腺的病理变化以及NF-κB通路的重要功能。
