UNASSIGNED: We established a rat salivary gland duct ligation model and observed pathological changes in the rat submandibular gland on day 1 and weeks 1, 2, 3, and 4 using hematoxylin and eosin staining, Alcian blue-periodic acid Schiff staining, Masson staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), and immunohistochemical staining. RNA sequencing was performed on normal salivary glands and those from the ligation model after 1 week. Significantly differentially expressed genes were selected, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed.
UNASSIGNED: Apoptosis levels and histological and functional KEGG pathway analyses showed that injury to the salivary gland after ligation gradually increased. The TGF-β pathway was activated and promoted fibrosis. RNA sequencing results and further verification of samples at week 1 showed that the NF-κB pathway plays a vital role in salivary gland atrophy.
UNASSIGNED: Our results detailed the pathological changes in the submandibular gland after ligation and the important functions of the NF-κB pathway.
■我们建立了大鼠唾液腺导管结扎模型,并在第1天和第1、2、3和4周采用苏木精和伊红染色观察了大鼠下颌下腺的病理变化,阿尔辛蓝高碘酸希夫染色,Masson染色,末端脱氧核苷酸转移酶dUTP缺口末端标记(TUNEL),和免疫组织化学染色。在1周后对正常唾液腺和来自连接模型的唾液腺进行RNA测序。选择显著差异表达的基因,进行了基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径分析。
■细胞凋亡水平和组织学和功能KEGG通路分析表明,结扎后唾液腺的损伤逐渐增加。TGF-β途径被激活并促进纤维化。RNA测序结果和第1周样本的进一步验证表明,NF-κB通路在唾液腺萎缩中起着至关重要的作用。
■我们的结果详细介绍了结扎后颌下腺的病理变化以及NF-κB通路的重要功能。