PDF(Pubmed)
Abstract：
UNASSIGNED: Matrix metalloproteinase 7 (MMP7) has been suggested as a promising biomarker in diagnosing biliary atresia (BA). This study aimed to assess the diagnostic accuracy of serum MMP7 in BA in the Middle Eastern population.
UNASSIGNED: In this cross-sectional study, neonates and infants with direct hyperbilirubinemia admitted to Namazi referral hospital, Shiraz, Iran, were studied. Baseline demographic and clinical characteristics and blood samples were obtained on admission. MMP7 serum concentration was measured using an enzyme-linked immunosorbent assay (ZellBio GmbH, Ulm, Germany).
UNASSIGNED: 44 infants with a mean age of 65.59 days were studied. Of these patients, 13 cases were diagnosed with BA, and 31 cases\' cholestasis related to other etiologies. Serum MMP7 concertation was 2.13 ng/mL in the BA group and 1.85 ng/mL in the non-BA group. MMP7 was significantly higher in those presented with either dark urine or acholic stool. The predictive performance capability of the MMP7 was not significant in the discrimination of BA from the non-BA group based on receiver operating characteristic curve analysis (area under curve: 0.6, 95% confidence interval: 0.45-0.75). In the optimal cut of point 1.9, the sensitivity and specificity were 84.6% and 45.1%, respectively. Further combination of MMP7 with Gamma-glutamyl transferase (GGT), alkaline phosphatase, direct and total bilirubin, and dark urine or acholic stool was not remarkably boosted the diagnostic accuracy of the test. Interestingly, GGT at a cut-off point of 230 U/L was 84.6% sensitive and 90.3% specific for BA.
UNASSIGNED: Our results are not consistent with previous studies on this subject. Considering more conventional and available tests like GGT besides conducting future studies with greater samples and different geographical areas is recommended.
UNASSIGNED: In this cross-sectional study, neonates and infants with direct hyperbilirubinemia admitted to Namazi referral hospital, Shiraz, Iran, were studied. Baseline demographic and clinical characteristics and blood samples were obtained on admission. MMP7 serum concentration was measured using an enzyme-linked immunosorbent assay (ZellBio GmbH, Ulm, Germany).
UNASSIGNED: 44 infants with a mean age of 65.59 days were studied. Of these patients, 13 cases were diagnosed with BA, and 31 cases\' cholestasis related to other etiologies. Serum MMP7 concertation was 2.13 ng/mL in the BA group and 1.85 ng/mL in the non-BA group. MMP7 was significantly higher in those presented with either dark urine or acholic stool. The predictive performance capability of the MMP7 was not significant in the discrimination of BA from the non-BA group based on receiver operating characteristic curve analysis (area under curve: 0.6, 95% confidence interval: 0.45-0.75). In the optimal cut of point 1.9, the sensitivity and specificity were 84.6% and 45.1%, respectively. Further combination of MMP7 with Gamma-glutamyl transferase (GGT), alkaline phosphatase, direct and total bilirubin, and dark urine or acholic stool was not remarkably boosted the diagnostic accuracy of the test. Interestingly, GGT at a cut-off point of 230 U/L was 84.6% sensitive and 90.3% specific for BA.
UNASSIGNED: Our results are not consistent with previous studies on this subject. Considering more conventional and available tests like GGT besides conducting future studies with greater samples and different geographical areas is recommended.
摘要：
■基质金属蛋白酶7（MMP7）已被认为是诊断胆道闭锁（BA）的有希望的生物标志物。本研究旨在评估中东人群BA中血清MMP7的诊断准确性。
■在这项横断面研究中，纳马齐转诊医院收治的直接高胆红素血症新生儿和婴儿，设拉子,伊朗,被研究过。入院时获得基线人口统计学和临床特征以及血液样本。使用酶联免疫吸附测定法测量MMP7血清浓度(ZellBioGmbH，乌尔姆,德国)。
■研究了44名平均年龄为65.59天的婴儿。在这些病人中,13例确诊为BA，与其他病因有关的胆汁淤积31例。BA组血清MMP7浓度为2.13ng/mL，非BA组为1.85ng/mL。MMP7在那些有深色尿液或大便的人中明显更高。基于受试者工作特征曲线分析(曲线下面积:0.6,95%置信区间:0.45-0.75),MMP7的预测性能能力在BA与非BA组的区分中不显著。在1.9点的最佳切割中，灵敏度和特异度分别为84.6%和45.1%，分别。MMP7与γ-谷氨酰转移酶（GGT）的进一步组合，碱性磷酸酶,直接和总胆红素，深色尿液或下大便没有显着提高测试的诊断准确性。有趣的是,在230U/L的截止点下，GGT对BA的敏感性为84.6％，特异性为90.3％。
■我们的结果与以前关于该主题的研究不一致。考虑到更多的常规和可用的测试，如GGT，除了进行未来的研究与更大的样本和不同的地理区域建议。
■在这项横断面研究中，纳马齐转诊医院收治的直接高胆红素血症新生儿和婴儿，设拉子,伊朗,被研究过。入院时获得基线人口统计学和临床特征以及血液样本。使用酶联免疫吸附测定法测量MMP7血清浓度(ZellBioGmbH，乌尔姆,德国)。
■研究了44名平均年龄为65.59天的婴儿。在这些病人中,13例确诊为BA，与其他病因有关的胆汁淤积31例。BA组血清MMP7浓度为2.13ng/mL，非BA组为1.85ng/mL。MMP7在那些有深色尿液或大便的人中明显更高。基于受试者工作特征曲线分析(曲线下面积:0.6,95%置信区间:0.45-0.75),MMP7的预测性能能力在BA与非BA组的区分中不显著。在1.9点的最佳切割中，灵敏度和特异度分别为84.6%和45.1%，分别。MMP7与γ-谷氨酰转移酶（GGT）的进一步组合，碱性磷酸酶,直接和总胆红素，深色尿液或下大便没有显着提高测试的诊断准确性。有趣的是,在230U/L的截止点下，GGT对BA的敏感性为84.6％，特异性为90.3％。
■我们的结果与以前关于该主题的研究不一致。考虑到更多的常规和可用的测试，如GGT，除了进行未来的研究与更大的样本和不同的地理区域建议。
