Abstract：
Engineered electroactive bacteria have potential applications ranging from sensing to biosynthesis. In order to advance the use of engineered electroactive bacteria, it is important to demonstrate functional expression of electron transfer modules in chassis adapted to operationally relevant conditions, such as non-freshwater environments. Here, we use the Shewanella oneidensis electron transfer pathway to induce current production in a marine bacterium, Marinobacter atlanticus, during biofilm growth in artificial seawater. Genetically encoded sensors optimized for use in Escherichia coli were used to control protein expression in planktonic and biofilm attached cells. Significant current production required the addition of menaquinone, which M. atlanticus does not produce, for electron transfer from the inner membrane to the expressed electron transfer pathway. Current through the S. oneidensis pathway in M. atlanticus was observed when inducing molecules were present during biofilm formation. Electron transfer was also reversible, indicating that electron transfer into M. atlanticus could be controlled. These results show that an operationally relevant marine bacterium can be genetically engineered for environmental sensing and response using an electrical signal.
摘要：
工程化的电活性细菌具有从传感到生物合成的潜在应用。为了推进工程电活性细菌的使用，重要的是演示电子转移模块在机箱中适应操作相关条件的功能表达，如非淡水环境。这里,我们使用Shewanellaoneidensis电子转移途径在海洋细菌中诱导电流产生，大西洋马氏杆菌,在人工海水中生物膜生长过程中。优化用于大肠杆菌的遗传编码传感器用于控制浮游和生物膜附着细胞中的蛋白质表达。目前大量生产需要添加甲基萘醌，大西洋M.不生产，用于从内膜到表达的电子转移途径的电子转移。当在生物膜形成过程中存在诱导分子时，观察到通过Atlandicus中的S.oneidensis途径的电流。电子转移也是可逆的，这表明电子转移到Atlanticus.这些结果表明，可以对操作相关的海洋细菌进行基因工程改造，以使用电信号进行环境感知和响应。
