Abstract：
BACKGROUND: Accumulating evidence has shown that gut microbiota plays a key role in the progression of atopic dermatitis (AD). Fecal microbiota transplantation (FMT), as an effective method to restore gut microbiota homeostasis, has been successfully applied for treating many inflammatory diseases. However, the therapeutic effect of FMT on AD remains unclear. The following study examined the effect and mechanism of FMT on AD-skin lesions in an AD mouse model.
METHODS: In this study, we exposed the shaved back skin of BALB/c mice to calcipotriol (MC903) to induce AD model. Mice were then treated with FMT, which was performed with gut microbiota from healthy mice. The gut microbiota of treated mice was tracked by 16S rRNA gene sequencing. Mice skin tissues were examined by histopathology and inflammatory cytokines change in serum by ELISA.
RESULTS: FMT had a faster trend on the reversion of the increases in skin epidermal layer thicknesses and suppressed some of the representative inflammatory cytokines. The gut microbial community in the natural recovery process varied significantly in the FMT group at day 7 (ANOSIM P = 0.0229, r = 0.2593). Notably, FMT had a long-lasting and beneficial impact on the gut microbial compositions of AD mice by increasing the ratio of Firmicutes to Bacteroidetes and the amount of butyric-producing bacteria (BPB), including Erysipelotrichaceae, Lactobacillaceae, and Eubacteriacea. Furthermore, the relative abundances of gut microbiota-mediated functional pathways involved in the cell growth and death, amino acid, energy, lipid, and carbohydrate metabolisms, and immune system increased after FMT treatment.
CONCLUSIONS: FMT modulated the gut microbiota homeostasis and affected the recovery from AD-related inflammations, suggesting that it could be used as a treatment strategy for AD patients in the clinic.
METHODS: In this study, we exposed the shaved back skin of BALB/c mice to calcipotriol (MC903) to induce AD model. Mice were then treated with FMT, which was performed with gut microbiota from healthy mice. The gut microbiota of treated mice was tracked by 16S rRNA gene sequencing. Mice skin tissues were examined by histopathology and inflammatory cytokines change in serum by ELISA.
RESULTS: FMT had a faster trend on the reversion of the increases in skin epidermal layer thicknesses and suppressed some of the representative inflammatory cytokines. The gut microbial community in the natural recovery process varied significantly in the FMT group at day 7 (ANOSIM P = 0.0229, r = 0.2593). Notably, FMT had a long-lasting and beneficial impact on the gut microbial compositions of AD mice by increasing the ratio of Firmicutes to Bacteroidetes and the amount of butyric-producing bacteria (BPB), including Erysipelotrichaceae, Lactobacillaceae, and Eubacteriacea. Furthermore, the relative abundances of gut microbiota-mediated functional pathways involved in the cell growth and death, amino acid, energy, lipid, and carbohydrate metabolisms, and immune system increased after FMT treatment.
CONCLUSIONS: FMT modulated the gut microbiota homeostasis and affected the recovery from AD-related inflammations, suggesting that it could be used as a treatment strategy for AD patients in the clinic.
摘要：
背景：越来越多的证据表明，肠道菌群在特应性皮炎（AD）的进展中起着关键作用。粪便微生物移植（FMT），作为恢复肠道菌群稳态的有效方法，已成功应用于多种炎症性疾病的治疗。然而,FMT对AD的治疗效果尚不清楚.以下研究检查了FMT对AD小鼠模型中的AD皮肤损伤的作用和机制。
方法：在本研究中，我们将BALB/c小鼠剃毛的背部皮肤暴露于卡泊三醇（MC903）以诱导AD模型。然后用FMT治疗小鼠，这是用健康小鼠的肠道微生物群进行的。通过16SrRNA基因测序追踪治疗小鼠的肠道微生物群。通过组织病理学和ELISA检测小鼠皮肤组织中炎症细胞因子的变化。
结果：FMT对皮肤表皮层厚度的增加有更快的逆转趋势，并抑制了一些代表性的炎性细胞因子。FMT组在第7天自然恢复过程中的肠道微生物群落差异显着（ANOSIMP=0.0229，r=0.2593）。值得注意的是,FMT通过增加Firmicutes与拟杆菌的比例和产生丁酸的细菌（BPB）的数量，对AD小鼠的肠道微生物组成有长期持久和有益的影响。包括丹毒草科，乳酸杆菌科，和Eubacteriacea.此外，涉及细胞生长和死亡的肠道微生物群介导的功能途径的相对丰富，氨基酸,能源,脂质，和碳水化合物代谢，FMT治疗后免疫系统增强。
结论：FMT调节肠道菌群稳态并影响AD相关炎症的恢复，这表明它可以在临床上用作AD患者的治疗策略。
方法：在本研究中，我们将BALB/c小鼠剃毛的背部皮肤暴露于卡泊三醇（MC903）以诱导AD模型。然后用FMT治疗小鼠，这是用健康小鼠的肠道微生物群进行的。通过16SrRNA基因测序追踪治疗小鼠的肠道微生物群。通过组织病理学和ELISA检测小鼠皮肤组织中炎症细胞因子的变化。
结果：FMT对皮肤表皮层厚度的增加有更快的逆转趋势，并抑制了一些代表性的炎性细胞因子。FMT组在第7天自然恢复过程中的肠道微生物群落差异显着（ANOSIMP=0.0229，r=0.2593）。值得注意的是,FMT通过增加Firmicutes与拟杆菌的比例和产生丁酸的细菌（BPB）的数量，对AD小鼠的肠道微生物组成有长期持久和有益的影响。包括丹毒草科，乳酸杆菌科，和Eubacteriacea.此外，涉及细胞生长和死亡的肠道微生物群介导的功能途径的相对丰富，氨基酸,能源,脂质，和碳水化合物代谢，FMT治疗后免疫系统增强。
结论：FMT调节肠道菌群稳态并影响AD相关炎症的恢复，这表明它可以在临床上用作AD患者的治疗策略。
