Abstract：
To determine the environmental persistence of Nannizziopsis guarroi on clinically relevant solid and aqueous substrates.
2 molecularly confirmed isolates of N guarroi obtained from clinical cases of dermatomycosis in bearded dragons (Pogona vitticeps).
3 concentrations (1 McFarland, 1:10 McFarland, and 1:100 McFarland) of fungal suspension were exposed to 7 sterilized solid substrates (fabric aquarium liner, wood mulch, sand, hard plastic, glass, cotton, and stainless steel) and 2 sterilized aqueous substrates (distilled water, saline solution [0.9% NaCl]). Biological replicates were performed for the contamination of the solid substrates. On days 1, 3, and 14 after contamination, the substrates were sampled for fungal culture with technical repeat. Fungal cultures were incubated at room temperature for 10 days and then evaluated for fungal growth.
Data from wood mulch were not evaluated because of plate contamination. Overall, the ability to culture N guarroi from solid substrates was isolate, time, and fungal concentration dependent. Viable fungus was isolated from fabric aquarium liner and glass on day 1 and days 1 and 3, respectively. N guarroi was cultured from all other solid substrates at day 14 from at least 1 isolate and/or fungal concentration. Viable N guarroi was isolated from both aqueous substrates at day 14, regardless of isolate or fungal concentration.
The environmental persistence of N guarroi should be considered when treating lizards infected with this fungus. Fomites may contribute to the contagious nature of this pathogen and environmental disinfection should be performed to reduce transmission.
2 molecularly confirmed isolates of N guarroi obtained from clinical cases of dermatomycosis in bearded dragons (Pogona vitticeps).
3 concentrations (1 McFarland, 1:10 McFarland, and 1:100 McFarland) of fungal suspension were exposed to 7 sterilized solid substrates (fabric aquarium liner, wood mulch, sand, hard plastic, glass, cotton, and stainless steel) and 2 sterilized aqueous substrates (distilled water, saline solution [0.9% NaCl]). Biological replicates were performed for the contamination of the solid substrates. On days 1, 3, and 14 after contamination, the substrates were sampled for fungal culture with technical repeat. Fungal cultures were incubated at room temperature for 10 days and then evaluated for fungal growth.
Data from wood mulch were not evaluated because of plate contamination. Overall, the ability to culture N guarroi from solid substrates was isolate, time, and fungal concentration dependent. Viable fungus was isolated from fabric aquarium liner and glass on day 1 and days 1 and 3, respectively. N guarroi was cultured from all other solid substrates at day 14 from at least 1 isolate and/or fungal concentration. Viable N guarroi was isolated from both aqueous substrates at day 14, regardless of isolate or fungal concentration.
The environmental persistence of N guarroi should be considered when treating lizards infected with this fungus. Fomites may contribute to the contagious nature of this pathogen and environmental disinfection should be performed to reduce transmission.
摘要：
目的：确定Nannizziopsisguarroi在临床相关固体和水性底物上的环境持久性。
方法：2个分子确认的从有胡子龙（Pogonavitticeps）的皮肤真菌病临床病例中获得的Nguarroi分离株。
方法：3个浓度（1McFarland，1:10麦克法兰，和1：100McFarland）的真菌悬浮液暴露于7个灭菌的固体基质（织物水族馆衬垫，木材覆盖物,沙子,硬质塑料，玻璃,棉花,和不锈钢）和2个灭菌的水性基质（蒸馏水，盐溶液[0.9%NaCl])。对固体基质的污染进行生物复制。在污染后的第1、3和14天，对底物进行技术重复的真菌培养取样。将真菌培养物在室温下孵育10天,然后评价真菌生长。
结果：由于平板污染，未评估来自木材覆盖物的数据。总的来说,从固体基质中培养Nguarroi的能力被分离，时间,和真菌浓度依赖性。分别在第1天以及第1天和第3天从织物水族馆衬里和玻璃中分离出活菌。在第14天从至少1个分离物和/或真菌浓度的所有其他固体基质中培养Nguarroi。无论分离物或真菌浓度如何，在第14天从两种水性底物中分离出活菌Nguarroi。
结论：在治疗感染了这种真菌的蜥蜴时，应考虑Nguarroi的环境持久性。Fomites可能导致该病原体的传染性，应进行环境消毒以减少传播。
方法：2个分子确认的从有胡子龙（Pogonavitticeps）的皮肤真菌病临床病例中获得的Nguarroi分离株。
方法：3个浓度（1McFarland，1:10麦克法兰，和1：100McFarland）的真菌悬浮液暴露于7个灭菌的固体基质（织物水族馆衬垫，木材覆盖物,沙子,硬质塑料，玻璃,棉花,和不锈钢）和2个灭菌的水性基质（蒸馏水，盐溶液[0.9%NaCl])。对固体基质的污染进行生物复制。在污染后的第1、3和14天，对底物进行技术重复的真菌培养取样。将真菌培养物在室温下孵育10天,然后评价真菌生长。
结果：由于平板污染，未评估来自木材覆盖物的数据。总的来说,从固体基质中培养Nguarroi的能力被分离，时间,和真菌浓度依赖性。分别在第1天以及第1天和第3天从织物水族馆衬里和玻璃中分离出活菌。在第14天从至少1个分离物和/或真菌浓度的所有其他固体基质中培养Nguarroi。无论分离物或真菌浓度如何，在第14天从两种水性底物中分离出活菌Nguarroi。
结论：在治疗感染了这种真菌的蜥蜴时，应考虑Nguarroi的环境持久性。Fomites可能导致该病原体的传染性，应进行环境消毒以减少传播。
