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Abstract：
The yeast Vanrija (previously Cryptococcus) humicola strain UJ1 produces d-aspartate oxidase (DDO) only in the presence of d-aspartate in culture media. This article provides RNA-sequencing data to identify the differentially expressed genes (DEGs) in the yeast cells grown between l- and d-aspartate. RNA samples were prepared from the yeast cells grown in a culture medium containing 30 mM d-aspartate or l-aspartate as the sole carbon source and subjected to RNA sequencing on Illumina NovaSeq6000 platform. The clean reads obtained by removing adaptor sequences and low-quality reads from raw reads were submitted to the Sequence Read Archive (SRA) database of the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJDB13570. The clean reads were subjected to differential gene expression analysis using DEGSeq to provide data on the upregulated and downregulated DEGs in the cells grown on d-aspartate. The DEGs were subjected to gene ontology (GO) and KEGG pathway enrichment analyses using GOSeq and KOBAS, respectively, to provide data on the possible biological functions of the DEGs. The data set obtained in this project might be helpful for further investigation of the effects of d-aspartate on cellular processes in yeast cells and other eukaryotic organisms.
摘要：
酵母Vanrija（以前是隐球菌）humicola菌株UJ1仅在培养基中存在d-天冬氨酸的情况下产生d-天冬氨酸氧化酶（DDO）。本文提供RNA测序数据以鉴定在1-和d-天冬氨酸之间生长的酵母细胞中的差异表达基因(DEG)。从在含有30mMd-天冬氨酸或1-天冬氨酸作为唯一碳源的培养基中生长的酵母细胞制备RNA样品,并在IlluminaNovaSeq6000平台上进行RNA测序。通过从原始读段中去除衔接子序列和低质量读段获得的干净读段以BioProject登录号PRJDB13570提交到国家生物技术信息中心(NCBI)的序列读段存档(SRA)数据库。使用DEGSeq对干净的读数进行差异基因表达分析,以提供关于在d-天冬氨酸上生长的细胞中上调和下调的DEG的数据。使用GOSeq和KOBAS对DEGs进行基因本体论（GO）和KEGG途径富集分析，分别,提供有关DEGs可能的生物学功能的数据。本项目中获得的数据集可能有助于进一步研究d-天冬氨酸对酵母细胞和其他真核生物细胞过程的影响。
