Abstract：
Food allergy (FA) has become a global food safety issue. Evidence suggests that inflammatory bowel disease (IBD) can increase the incidence of FA, but it is mostly based on epidemiological studies. An animal model is pivotal for unraveling the mechanisms involved. However, dextran sulfate sodium (DSS)-induced IBD models may cause substantial animal losses. To better investigate the effect of IBD on FA, this study aimed to establish a murine model to fit both IBD and FA symptoms. Firstly, we compared three DSS-induced colitis models by monitoring survival rate, disease activity index, colon length, and spleen index, and then eliminated the colitis model with a 7-day administration of 4% due to high mortality. Moreover, we evaluated the modeling effects on FA and intestinal histopathology of the two models selected and found the modeling effects were similar in both the colitis model with a 7-day administration of 3% DSS and the colitis model with long-term administration of DSS. However, for animal survival reasons, we recommend the colitis model with long-term administration of DSS.
摘要：
食物过敏(FA)已成为全球性的食品安全问题。有证据表明，炎症性肠病（IBD）可以增加FA的发病率，但它主要是基于流行病学研究。动物模型对于揭示所涉及的机制至关重要。然而,葡聚糖硫酸钠（DSS）诱导的IBD模型可能会导致大量动物损失。为了更好地研究IBD对FA的影响，本研究旨在建立一个符合IBD和FA症状的小鼠模型。首先,我们通过监测生存率比较了三种DSS诱导的结肠炎模型，疾病活动指数，结肠长度,和脾脏指数，然后用4％的7天给药消除结肠炎模型，因为死亡率高。此外，我们评估了所选择的两种模型对FA和肠组织病理学的建模效果,发现3%DSS给药7天的结肠炎模型和DSS长期给药的结肠炎模型的建模效果相似.然而,为了动物生存的原因，我们推荐长期使用DSS的结肠炎模型.
