Abstract：
This study aimed to provide information on the presence and frequency of viral and parasitic agents in wildlife presented to a Veterinary Teaching Hospital in 2020-2021. Serum and faecal samples were collected from 50 rescued animals (roe deer, fallow deer, foxes, badgers, pine martens, and porcupines) and examined by serological, molecular, and parasitological techniques. Transtracheal wash (TTW) was also collected post-mortem from roe deer. Overall, the results of the different techniques showed infections with the following viral and parasitic agents: Bovine Viral Diarrhea Virus, Small Ruminant Lentiviruses, Kobuvirus, Astrovirus, Canine Adenovirus 1, Bopivirus, gastrointestinal strongyles, Capillaria, Ancylostomatidae, Toxocara canis, Trichuris vulpis, Hymenolepis, Strongyloides, Eimeria, Isospora, Dictyocaulus, Angiostrongylus vasorum, Crenosoma, Dirofilaria immitis, Neospora caninum, Giardia duodenalis, and Cryptosporidium. Sequencing (Tpi locus) identified G. duodenalis sub-assemblages AI and BIV in one roe deer and one porcupine, respectively. Adult lungworms collected from the TTW were identified as Dictyocaulus capreolus (COX1 gene). This is the first molecular identification of G. duodenalis sub-assemblage AI and D. capreolus in roe deer in Italy. These results show a wide presence of pathogens in wild populations and provide an overview of environmental health surveillance.
摘要：
这项研究旨在提供有关2020-2021年提交给兽医教学医院的野生动物中病毒和寄生虫的存在和频率的信息。从50只获救的动物中收集血清和粪便样本(ro，休养鹿,狐狸,badgers,松树马,和豪猪)，并通过血清学检查，分子，和寄生虫学技术。死后还从the中收集了气管冲洗液（TTW）。总的来说,不同技术的结果显示感染以下病毒和寄生虫：牛病毒性腹泻病毒，小反刍动物慢病毒,Kobuvirus,星状病毒,犬腺病毒1型,Bopivirus,胃肠强壮，毛细管,假单胞菌科，犬弓形虫,毛癣菌,处女膜外膜,类圆线虫,艾美球虫,Isospora,Dictyocaulus,血管管圆线虫,Crenosoma,Dirofilariaimmitis，新孢子虫,十二指肠贾第虫,和隐孢子虫.测序（Tpi基因座）确定了一只roe和一只豪猪中的G.daudenalis亚组合AI和BIV，分别。从TTW中收集的成年肺虫被鉴定为网纹毛虫（COX1基因）。这是对意大利the中的G.daudenalis亚组合AI和D.capreolus的首次分子鉴定。这些结果表明，在野生种群中广泛存在病原体，并提供了环境卫生监测的概述。
