Abstract：
Developing sound breeding programs for aquaculture species may be challenging when matings cannot be controlled due to communal spawning. We developed a genotyping-by-sequencing marker panel of 300 SNPs for parentage testing and sex determination by using data from an in-house reference genome as well as a 90 K SNP genotyping array based on different populations of yellowtail kingfish (Seriola lalandi). The minimum and maximum distance between adjacent marker pairs were 0.7 Mb and 13 Mb, respectively, with an average marker spacing of 2 Mb. Weak evidence of the linkage disequilibrium between adjacent marker pairs was found. The results showed high panel performance for parental assignment, with probability exclusion values equaling 1. The rate of false positives when using cross-population data was null. A skewed distribution of genetic contributions by dominant females was observed, thus increasing the risk of higher rates of inbreeding in subsequent captive generations when no parentage data are used. All these results are discussed in the context of breeding program design, using this marker panel to increase the sustainability of this aquaculture resource.
摘要：
当由于公共产卵而无法控制交配时，为水产养殖物种开发合理的育种计划可能具有挑战性。通过使用内部参考基因组的数据以及基于不同种群的黄尾金鱼（Seriolalalandi）的90KSNP基因分型阵列，我们开发了300个SNP的基因分型标记组，用于亲子关系测试和性别确定。相邻标记对之间的最小和最大距离分别为0.7Mb和13Mb，分别,平均标记间距为2Mb。发现了相邻标记对之间连锁不平衡的弱证据。结果显示，家长分配的面板性能很高，概率排除值等于1。使用交叉群体数据时的假阳性率为null。观察到显性雌性的遗传贡献的偏斜分布，因此，当没有使用亲子关系数据时，在随后的圈养世代中近亲繁殖率增加的风险。所有这些结果都在育种程序设计的背景下进行了讨论，使用这个标记面板来增加这种水产养殖资源的可持续性。
