PDF(Pubmed)
Abstract：
UNASSIGNED: Silkworm products were first used by physicians more than 8500 years ago, in the early Neolithic period. In Persian medicine, silkworm extract has several uses for treating and preventing neurological, cardiac, and liver diseases. Mature silkworms (Bombyx mori) and their pupae contain a variety of growth factors and proteins that can be used in many repair processes, including nerve regeneration.
UNASSIGNED: The study aimed to evaluate the effects of mature silkworm (Bombyx mori), and silkworm pupae extract on Schwann cell proliferation and axon growth.
UNASSIGNED: Silkworm (Bombyx mori) and silkworm pupae extracts were prepared. Then, the concentration and type of amino acids and proteins in the extracts were evaluated by Bradford assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and liquid chromatograph-mass spectrometer (LC-MS/MS). Also, the regenerative potential of extracts for improving Schwann cell proliferation and axon growth was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, electron microscopy, and NeuroFilament-200 (NF-200) immunostaining.
UNASSIGNED: According to the results of the Bradford test, the total protein content of pupae extract was almost twice that of mature worm extract. Also, SDS-PAGE analysis revealed numerous proteins and growth factors, such as bombyrin and laminin, in extracts that are involved in the repair of the nervous system. In accordance with Bradford\'s results, the evaluation of extracts using LC-MS/MS revealed that the number of amino acids in pupae extract was higher than in mature silkworm extract. It was found that the proliferation of Schwann cells at a concentration of 0.25 mg/mL in both extracts was higher than the concentrations of 0.01 and 0.05 mg/mL. When using both extracts on dorsal root ganglion (DRGs), an increase in length and number was observed in axons.
UNASSIGNED: The findings of this study demonstrated that extracts obtained from silkworms, especially pupae, can play an effective role in Schwann cell proliferation and axonal growth, which can be strong evidence for nerve regeneration, and, consequently, repairing peripheral nerve damage.
UNASSIGNED: The study aimed to evaluate the effects of mature silkworm (Bombyx mori), and silkworm pupae extract on Schwann cell proliferation and axon growth.
UNASSIGNED: Silkworm (Bombyx mori) and silkworm pupae extracts were prepared. Then, the concentration and type of amino acids and proteins in the extracts were evaluated by Bradford assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and liquid chromatograph-mass spectrometer (LC-MS/MS). Also, the regenerative potential of extracts for improving Schwann cell proliferation and axon growth was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, electron microscopy, and NeuroFilament-200 (NF-200) immunostaining.
UNASSIGNED: According to the results of the Bradford test, the total protein content of pupae extract was almost twice that of mature worm extract. Also, SDS-PAGE analysis revealed numerous proteins and growth factors, such as bombyrin and laminin, in extracts that are involved in the repair of the nervous system. In accordance with Bradford\'s results, the evaluation of extracts using LC-MS/MS revealed that the number of amino acids in pupae extract was higher than in mature silkworm extract. It was found that the proliferation of Schwann cells at a concentration of 0.25 mg/mL in both extracts was higher than the concentrations of 0.01 and 0.05 mg/mL. When using both extracts on dorsal root ganglion (DRGs), an increase in length and number was observed in axons.
UNASSIGNED: The findings of this study demonstrated that extracts obtained from silkworms, especially pupae, can play an effective role in Schwann cell proliferation and axonal growth, which can be strong evidence for nerve regeneration, and, consequently, repairing peripheral nerve damage.
摘要：
未经授权：家蚕产品在8500多年前首次被医生使用，在新石器时代早期。在波斯医学中，家蚕提取物有多种治疗和预防神经系统的用途，心脏,和肝脏疾病。成熟蚕(Bombyxmori)及其蛹含有多种生长因子和蛋白质,可用于多种修复过程,包括神经再生.
UnASSIGNED：该研究旨在评估成熟的家蚕（Bombyxmori）的影响，和蚕蛹提取物对许旺细胞增殖和轴突生长的影响。
未经授权:制备家蚕(Bombyxmori)和蚕蛹提取物。然后，通过Bradford测定法评估提取物中氨基酸和蛋白质的浓度和类型，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),和液相色谱-质谱联用仪(LC-MS/MS)。此外，通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（MTT）测定法检查提取物改善雪旺细胞增殖和轴突生长的再生潜力，电子显微镜，和Neurofilament-200(NF-200)免疫染色。
UNASSIGNED：根据布拉德福德测试的结果，p提取物的总蛋白含量几乎是成熟蠕虫提取物的两倍。此外，SDS-PAGE分析揭示了许多蛋白质和生长因子，比如bombyrin和层粘连蛋白，参与神经系统修复的提取物。根据布拉德福德的结果,用LC-MS/MS对提取物进行评估，发现p提取物中的氨基酸数量高于成熟的家蚕提取物。发现两种提取物中0.25mg/mL浓度的施万细胞的增殖高于0.01和0.05mg/mL的浓度。当在背根神经节（DRGs）上使用两种提取物时，在轴突中观察到长度和数量的增加。
UNASSIGNED：这项研究的结果表明，从蚕中获得的提取物，尤其是蛹,可以在雪旺细胞增殖和轴突生长中发挥有效的作用，这可能是神经再生的有力证据，and,因此,修复周围神经损伤。
UnASSIGNED：该研究旨在评估成熟的家蚕（Bombyxmori）的影响，和蚕蛹提取物对许旺细胞增殖和轴突生长的影响。
未经授权:制备家蚕(Bombyxmori)和蚕蛹提取物。然后，通过Bradford测定法评估提取物中氨基酸和蛋白质的浓度和类型，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),和液相色谱-质谱联用仪(LC-MS/MS)。此外，通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（MTT）测定法检查提取物改善雪旺细胞增殖和轴突生长的再生潜力，电子显微镜，和Neurofilament-200(NF-200)免疫染色。
UNASSIGNED：根据布拉德福德测试的结果，p提取物的总蛋白含量几乎是成熟蠕虫提取物的两倍。此外，SDS-PAGE分析揭示了许多蛋白质和生长因子，比如bombyrin和层粘连蛋白，参与神经系统修复的提取物。根据布拉德福德的结果,用LC-MS/MS对提取物进行评估，发现p提取物中的氨基酸数量高于成熟的家蚕提取物。发现两种提取物中0.25mg/mL浓度的施万细胞的增殖高于0.01和0.05mg/mL的浓度。当在背根神经节（DRGs）上使用两种提取物时，在轴突中观察到长度和数量的增加。
UNASSIGNED：这项研究的结果表明，从蚕中获得的提取物，尤其是蛹,可以在雪旺细胞增殖和轴突生长中发挥有效的作用，这可能是神经再生的有力证据，and,因此,修复周围神经损伤。
