PDF(Pubmed)
Abstract：
Seminal plasma cytokines are associated with fertility and reproductive health, but progressing their clinical utility is hampered by absence of reference data on concentration ranges of relevant cytokines in healthy men. We employed a systematic approach to assemble current evidence on the concentrations of immune regulatory cytokines present in seminal plasma (SP) of normozoospermic and/or fertile men and evaluated the impact of different platform methodologies for cytokine quantification.
A systematic literature search was performed utilising PubMed, Web of Science and Scopus. Databases were searched from inception until 30th June 2022 inclusive, using combinations of keywords pertaining to seminal fluid and cytokines, and was restricted to human participants. Original data with values reported as concentration of specific cytokines in SP of men clearly defined as fertile or normozoospermic were extracted from studies written in English.
A total of 3769 publications were initially identified, of which 118 fulfilled the eligibility criteria for inclusion. A total of 51 individual cytokines are detectable in SP of healthy men. The number of studies reporting on each cytokine range from 1 to >20. The reported concentrations for many cytokines linked with fertility status, including IL6, CXCL8/IL8, and TNFA, are highly variable between published studies. This is associated with the different immunoassay methodologies utilised and may be exacerbated by a lack of validation of assays to ensure suitability for SP assessment. Due to the large variation between studies, accurate reference ranges for healthy men cannot be determined from the published data.
The concentrations of cytokines and chemokines detected in SP is inconsistent and highly variable between studies and cohorts, limiting current capacity to define reference ranges for cytokine concentrations in fertile men. The lack of standardisation in methods used to process and store SP, and variation in platforms used to evaluate cytokine abundance, are factors contributing to the observed heterogeneity. To progress the clinical utility of SP cytokine analysis will require standardisation and validation of methodologies so that reference ranges for healthy fertile men can be defined.
A systematic literature search was performed utilising PubMed, Web of Science and Scopus. Databases were searched from inception until 30th June 2022 inclusive, using combinations of keywords pertaining to seminal fluid and cytokines, and was restricted to human participants. Original data with values reported as concentration of specific cytokines in SP of men clearly defined as fertile or normozoospermic were extracted from studies written in English.
A total of 3769 publications were initially identified, of which 118 fulfilled the eligibility criteria for inclusion. A total of 51 individual cytokines are detectable in SP of healthy men. The number of studies reporting on each cytokine range from 1 to >20. The reported concentrations for many cytokines linked with fertility status, including IL6, CXCL8/IL8, and TNFA, are highly variable between published studies. This is associated with the different immunoassay methodologies utilised and may be exacerbated by a lack of validation of assays to ensure suitability for SP assessment. Due to the large variation between studies, accurate reference ranges for healthy men cannot be determined from the published data.
The concentrations of cytokines and chemokines detected in SP is inconsistent and highly variable between studies and cohorts, limiting current capacity to define reference ranges for cytokine concentrations in fertile men. The lack of standardisation in methods used to process and store SP, and variation in platforms used to evaluate cytokine abundance, are factors contributing to the observed heterogeneity. To progress the clinical utility of SP cytokine analysis will require standardisation and validation of methodologies so that reference ranges for healthy fertile men can be defined.
摘要：
目的：精浆细胞因子与生育和生殖健康有关，但是由于缺乏健康男性相关细胞因子浓度范围的参考数据，因此阻碍了其临床应用的发展。我们采用了一种系统的方法来收集有关正常精子症和/或可育男性的精浆（SP）中存在的免疫调节细胞因子浓度的最新证据，并评估了不同平台方法对细胞因子定量的影响。
方法：使用PubMed进行了系统的文献检索，WebofScience,还有Scopus.数据库从开始到2022年6月30日进行搜索，包括在内，使用与精液和细胞因子相关的关键词的组合，仅限于人类参与者。从以英语编写的研究中提取了原始数据，其数值报告为明确定义为可育或正常精子症的男性精浆中特定细胞因子的浓度。
结果：最初确定了总共3769种出版物，其中118人符合入选资格标准。在健康男性的精浆中检测到总共51种单独的细胞因子。报告每种细胞因子的研究数量范围为1至>20。报道的许多细胞因子的浓度与生育状态有关，包括IL6、CXCL8/IL8和TNFA，在已发表的研究之间差异很大。这与所使用的不同免疫测定方法有关，并且可能因缺乏确保精浆评估适用性的测定验证而加剧。由于研究之间的差异很大，从公布的数据中无法确定健康男性的准确参考范围。
结论：在SP中检测到的细胞因子和趋化因子的浓度在研究和队列之间不一致且差异很大，限制电流容量以定义可育男性细胞因子浓度的参考范围。精浆加工和储存方法缺乏标准化，以及用于评估细胞因子丰度的平台的变化，是导致观察到的异质性的因素。为了提高精浆细胞因子分析的临床实用性，将需要标准化和方法学验证，以便可以定义健康可育男性的参考范围。本文受版权保护。保留所有权利。
方法：使用PubMed进行了系统的文献检索，WebofScience,还有Scopus.数据库从开始到2022年6月30日进行搜索，包括在内，使用与精液和细胞因子相关的关键词的组合，仅限于人类参与者。从以英语编写的研究中提取了原始数据，其数值报告为明确定义为可育或正常精子症的男性精浆中特定细胞因子的浓度。
结果：最初确定了总共3769种出版物，其中118人符合入选资格标准。在健康男性的精浆中检测到总共51种单独的细胞因子。报告每种细胞因子的研究数量范围为1至>20。报道的许多细胞因子的浓度与生育状态有关，包括IL6、CXCL8/IL8和TNFA，在已发表的研究之间差异很大。这与所使用的不同免疫测定方法有关，并且可能因缺乏确保精浆评估适用性的测定验证而加剧。由于研究之间的差异很大，从公布的数据中无法确定健康男性的准确参考范围。
结论：在SP中检测到的细胞因子和趋化因子的浓度在研究和队列之间不一致且差异很大，限制电流容量以定义可育男性细胞因子浓度的参考范围。精浆加工和储存方法缺乏标准化，以及用于评估细胞因子丰度的平台的变化，是导致观察到的异质性的因素。为了提高精浆细胞因子分析的临床实用性，将需要标准化和方法学验证，以便可以定义健康可育男性的参考范围。本文受版权保护。保留所有权利。
