UNASSIGNED: A total of 28 male Wistar rats were divided equally into four groups. The negative control group (N) received no treatment, while the positive control group (C) and the other two treatment groups (T1, T2) were induced with LPS for 6 h, followed by the application of topical polyethylene glycol (PEG) hydrogels for C group, 25 ppm EGCG hydrogels for T1 group and 75 ppm EGCG hydrogels for T2 group, before being filled with glass ionomer cement (GIC). After 24 h, PEG and EGCG were reapplied and refilled with GIC for 24 h. The pulp tissue samples were examined by means of immunohistochemistry (IHC) to identify TNF-α, MDA and CGRP expression. All the data obtained was analyzed with one-way analyses of variance (ANOVA) test.
UNASSIGNED: The T1 and T2 groups showed a significant decrease in TNF-α and CGRP expression compared to the control group, but there was no significant decrease in MDA in either group (P<0.05).
UNASSIGNED: Based on the results of this study, topical application of 75 ppm EGCG hydrogels to the tooth cavities with six hours of pulpal inflammation has the optimal result in reducing the expression of TNF-α and CGRP, but not of MDA.
UnASSIGNED:将28只雄性Wistar大鼠平均分为4组。阴性对照组(N)不接受治疗,阳性对照组(C)和其他两个治疗组(T1、T2)分别用LPS诱导6h,随后局部应用聚乙二醇(PEG)水凝胶用于C组,T1组的25ppmEGCG水凝胶和T2组的75ppmEGCG水凝胶,在填充玻璃离聚物水泥(GIC)之前。24小时后,重新应用PEG和EGCG并重新填充GIC24小时。通过免疫组织化学(IHC)检查牙髓组织样品以鉴定TNF-α,MDA和CGRP表达。用单向方差分析(ANOVA)检验分析获得的所有数据。
UNASSIGNED:与对照组相比,T1和T2组显示TNF-α和CGRP表达显着降低,两组MDA均无明显下降(P<0.05)。
UNASSIGNED:根据这项研究的结果,将75ppmEGCG水凝胶局部应用于牙髓炎症6小时的牙腔具有降低TNF-α和CGRP表达的最佳结果,但不是MDA。