Abstract：
Gastric cancer (GC) is a serious disease with high mortality and poor prognosis. It is known that tRNA halves play key roles in the progression of cancer. This study explored the function of the tRNA half tRF-41-YDLBRY73W0K5KKOVD in GC. Quantitative real-time reverse transcription-polymerase chain reaction was used to measure RNA levels. The level of tRF-41-YDLBRY73W0K5KKOVD in GC cells was regulated by its mimics or inhibitor. Cell proliferation was evaluated by using a Cell Counting Kit-8 and EdU cell proliferation assay. A Transwell assay was used to detect cell migration. Flow cytometry was used to measure cell cycle and apoptosis. The results showed that tRF-41-YDLBRY73W0K5KKOVD expression was decreased in GC cells and tissues. Functionally, overexpression of tRF-41-YDLBRY73W0K5KKOVD inhibited cell proliferation, reduced migration, repressed the cell cycle, and promoted cell apoptosis in GC cells. Based on RNA sequencing results and luciferase reporter assays, 3\'-phosphoadenosine-5\'-phosphosulfate synthase 2 (PAPSS2) was identified as a target gene of tRF-41-YDLBRY73W0K5KKOVD. These findings indicated that tRF-41-YDLBRY73W0K5KKOVD inhibited GC progression, suggesting that tRF-41-YDLBRY73W0K5KKOVD might be a potential therapeutic target in GC.
摘要：
胃癌（GC）是一种严重的疾病，死亡率高，预后差。已知tRNA一半在癌症的进展中起关键作用。本研究探讨了tRNA半tRF-41-YDLBRY73W0K5KKOVD在GC中的功能。定量实时逆转录聚合酶链反应用于测量RNA水平。GC细胞中tRF-41-YDLBRY73W0K5KKOVD的水平受其模拟物或抑制剂的调节。通过使用细胞计数试剂盒-8和EdU细胞增殖测定来评估细胞增殖。使用Transwell测定法来检测细胞迁移。流式细胞术用于测量细胞周期和凋亡。成果显示tRF-41-YDLBRY73W0K5KKOVD在GC细胞和组织中表达降低。功能上,过表达tRF-41-YDLBRY73W0K5KKOVD抑制细胞增殖，减少迁移，抑制了细胞周期，促进GC细胞凋亡。根据RNA测序结果和荧光素酶报告基因测定，3'-磷酸腺苷-5'-磷酸硫酸合酶2（PAPSS2）被鉴定为tRF-41-YDLBRY73W0K5KKOVD的靶基因。这些发现表明tRF-41-YDLBRY73W0K5KKOVD抑制GC进展，提示tRF-41-YDLBRY73W0K5KKOVD可能是GC的潜在治疗靶点。
