PDF(Pubmed)
Abstract：
American shad (Alosa sapidissima), introduced from the United States, has become one of the most expensive farmed fish in the aquatic product market of China. The shad reveals significant sexual dimorphism in growth and behaviors. For the study, five male-specific tags were identified in two-generation breeding populations of Alosa sapidissima and were verified by PCR amplification. Averages of 10,245,091 and 8,685,704 raw and enzyme reads were obtained by high-throughput sequencing of the 2b-RAD library, respectively. 301,022 unique tags were obtained from the sequences of twenty samples with sequencing depths of 0 to 500. Finally, 274,324 special tags and 29,327 SNPs were selected with a sequencing depth of 3 to 500. Eleven preliminary screening male-specific tags and three male heterogametic SNP loci were isolated. After verification by PCR amplification, five male-specific sequences of 27 bp located on chromosome 3 were screened out. Chromosome 3 could be assumed to be the sex chromosome of Alosa sapidissima. Sex-specific markers will provide invaluable and systematic animal germplasm resources to allow for the precise identification of neo-males for the all-female breeding of Alosa sapidissima in commercial aquaculture.
摘要：
美国沙德(Alosasapidissima)，从美国引进的，已成为中国水产品市场上最昂贵的养殖鱼类之一。shad在生长和行为中表现出明显的性二态性。对于这项研究，在Alosasapidissima的两代育种种群中鉴定出五个雄性特异性标签，并通过PCR扩增进行了验证。通过对2b-RAD文库的高通量测序获得了10,245,091和8,685,704个原始和酶读数的平均值，分别。从20个样品的序列获得301,022个独特标签,测序深度为0至500。最后,以3至500的测序深度选择274,324个特殊标签和29,327个SNP。分离出11个初步筛选的雄性特异性标签和3个雄性异抗SNP基因座。通过PCR扩增验证后，筛选出位于3号染色体上的5个27bp的雄性特异性序列。3号染色体可以假定为Alosasapidissima的性染色体。性别特异性标记将提供宝贵和系统的动物种质资源，以精确鉴定新雄性，用于商业水产养殖中Alosasapidissima的全雌性育种。
