Abstract：
The need to take sex into account in biomedical research is now recognized and mandated by funding institutions. In laboratory rodents, such as mice, sexing is usually performed anatomically or by genotyping using multiplex or simplex PCR techniques on genomic DNA. Here we present a simple RT-PCR-based method targeting Kdm5c and Kdm5d to determine genetic sex in mouse cDNA samples, allowing for retrospective sex determination.
摘要：
现在，资助机构认识到并授权在生物医学研究中考虑性别的必要性。在实验室啮齿动物中，比如老鼠，性别鉴定通常在解剖学上或通过使用多重或单纯形PCR技术对基因组DNA进行基因分型。在这里，我们提出了一种简单的基于RT-PCR的方法，靶向Kdm5c和Kdm5d来确定小鼠cDNA样品中的遗传性别，允许回顾性性别确定。
