PDF(Pubmed)
Abstract：
OBJECTIVE: Mammaglobin and GCDFP-15 are traditional immunohistochemistry (IHC) markers utilized to recognize metastasis of breast carcinoma in an unknown primary. GATA-3 is increasingly being used as a marker of primary breast origin. This study was done to evaluate and compare GATA-3 with GCDFP-15 and Mammaglobin in invasive primary including metastatic and triple negative breast carcinomas.
METHODS: Immunohistochemistry for GATA-3, GCDFP-15 and Mammaglobin was applied on 100 cases of primary breast carcinomas, including 20 triple negative cases and 30 cases of metastatic breast carcinomas. Staining scores were given for each marker by multiplying the percentage of positive tumor cells by the intensity of staining (1+, 2+ or 3+), with scores ranging from 0 to 300. Staining score of 1 or more was considered positive.
RESULTS: GATA-3 was expressed in 92% of primary, 80% of metastatic and 60% of triple negative breast carcinomas, with an average staining score of 270. Mammaglobin was expressed in 68% of primary, 56.6% of metastatic and 25% of triple negative breast carcinomas, with an average staining score of 180. GCDFP-15 was expressed in 48% of primary, 26.6% of metastatic and 05% of breast carcinomas, with an average staining score of 60. GATA-3 demonstrated to have higher staining score (average of 270) than other two markers in maximum number of cases.
CONCLUSIONS: GATA-3 has a higher sensitivity and increased staining scores in primary breast carcinomas, metastatic breast carcinomas as well as in triple negative breast carcinomas.
METHODS: Immunohistochemistry for GATA-3, GCDFP-15 and Mammaglobin was applied on 100 cases of primary breast carcinomas, including 20 triple negative cases and 30 cases of metastatic breast carcinomas. Staining scores were given for each marker by multiplying the percentage of positive tumor cells by the intensity of staining (1+, 2+ or 3+), with scores ranging from 0 to 300. Staining score of 1 or more was considered positive.
RESULTS: GATA-3 was expressed in 92% of primary, 80% of metastatic and 60% of triple negative breast carcinomas, with an average staining score of 270. Mammaglobin was expressed in 68% of primary, 56.6% of metastatic and 25% of triple negative breast carcinomas, with an average staining score of 180. GCDFP-15 was expressed in 48% of primary, 26.6% of metastatic and 05% of breast carcinomas, with an average staining score of 60. GATA-3 demonstrated to have higher staining score (average of 270) than other two markers in maximum number of cases.
CONCLUSIONS: GATA-3 has a higher sensitivity and increased staining scores in primary breast carcinomas, metastatic breast carcinomas as well as in triple negative breast carcinomas.
摘要：
目的：乳腺球蛋白和GCDFP-15是传统的免疫组织化学（IHC）标记，用于识别未知原发乳腺癌的转移。GATA-3越来越多地用作原发性乳腺起源的标记。进行这项研究是为了评估和比较GATA-3与GCDFP-15和乳腺浸润性原发癌，包括转移性和三阴性乳腺癌。
方法：对100例原发性乳腺癌患者应用GATA-3、GCDFP-15和乳腺球蛋白的免疫组织化学检测，包括20例三阴性病例和30例转移性乳腺癌。通过将阳性肿瘤细胞的百分比乘以染色强度（1+，2+或3+)，分数从0到300。染色评分为1或更高被认为是阳性的。
结果：GATA-3在92％的原发性中表达，80%的转移性乳腺癌和60%的三阴性乳腺癌，平均染色得分为270。乳腺球蛋白在原发灶的68%中表达，56.6%的转移性乳腺癌和25%的三阴性乳腺癌，平均染色评分为180。GCDFP-15在原发细胞中表达48%,26.6％的转移性乳腺癌和05％的乳腺癌，平均染色评分为60。GATA-3证明在最大数量的病例中具有比其他两种标记更高的染色评分(平均270)。
结论：GATA-3在原发性乳腺癌中具有更高的敏感性和更高的染色评分，转移性乳腺癌以及三阴性乳腺癌。
方法：对100例原发性乳腺癌患者应用GATA-3、GCDFP-15和乳腺球蛋白的免疫组织化学检测，
结果：GATA-3在92％的原发性中表达，
结论：GATA-3在原发性乳腺癌中具有更高的敏感性和更高的染色评分，
