UNASSIGNED: Prospective cohort study.
UNASSIGNED: This cohort study was conducted at Baptist Eye Institute, Kyoto, Japan, between October 2014 and October 2016. It included 68 patients with a 36-month follow-up period who had undergone successful Descemet stripping automated endothelial keratoplasty (DSAEK) or penetrating keratoplasty.
UNASSIGNED: Human CECs (HCECs) from remaining peripheral donor corneas were cultured and evaluated for maturity by surface markers (CD166+, CD44-/dull, CD24-, and CD105-) using fluorescence-activated cell sorting. Postoperative ECD was assessed according to the mature-differentiated HCEC contents: high-maturity group: > 70%, middle-maturity group: 10% to 70%, low-maturity group: < 10%. The successful rate of ECD maintained at 1500 cells/mm2 at 36 months postoperative was analyzed using the log-rank test.
UNASSIGNED: Endothelial cell density and ECL at 36 months postoperative.
UNASSIGNED: The 68 included patients (mean [standard deviation] age 68.1 [13.6] years, 47.1% women, 52.9% DSAEK). The high, middle, and low-maturity groups included 17, 32, and 19 eyes, respectively. At 36 months postoperative, the mean (standard deviation) ECD significantly decreased to 911 (388) cells/mm2 by 66% in the low-maturity group, compared with 1604 (436) by 40% and 1424 (613) cells/mm2 by 50% in the high and middle-maturity groups (P < 0.001 and P = 0.007, respectively) and the low-maturity group significantly failed to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001). Additional ECD analysis for patients who underwent DSAEK alone displayed a significant failure to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001).
UNASSIGNED: The high content of mature-differentiated HCECs expressed in culture by the donor peripheral cornea was coincident with low ECL, suggesting that a high-maturity CEC content predicts long-term graft survival. Understanding the molecular mechanism for maintaining HCEC maturity could elucidate the mechanism of ECL after corneal transplantation and aid in developing effective interventions.
UNASSIGNED: Proprietary or commercial disclosure may be found after the references.
未经评估:前瞻性队列研究。
未经评估:这项队列研究在浸信会眼科研究所进行,
UNASSIGNED:培养来自剩余外周供体角膜的人类CECs(HCECs),并通过表面标记(CD166,
未授权:术后36个月时的内皮细胞密度和ECL。
未经评估:68名患者(平均[标准差]年龄68.1[13.6]岁,
UASSIGNED:供体外周角膜在培养物中表达的成熟分化HCECs含量高,ECL低,
UNASSIGNED:在参考文献之后可以找到专有或商业披露。