PDF(Pubmed)
Abstract：
UNASSIGNED: Corneal endothelial cell density (ECD) gradually decreases after corneal transplantation by unknown biologic, biophysical, or immunologic mechanism. Our purpose was to assess the association between donor corneal endothelial cell (CEC) maturity in culture and postoperative endothelial cell loss (ECL) after successful corneal transplantation.
UNASSIGNED: Prospective cohort study.
UNASSIGNED: This cohort study was conducted at Baptist Eye Institute, Kyoto, Japan, between October 2014 and October 2016. It included 68 patients with a 36-month follow-up period who had undergone successful Descemet stripping automated endothelial keratoplasty (DSAEK) or penetrating keratoplasty.
UNASSIGNED: Human CECs (HCECs) from remaining peripheral donor corneas were cultured and evaluated for maturity by surface markers (CD166+, CD44-/dull, CD24-, and CD105-) using fluorescence-activated cell sorting. Postoperative ECD was assessed according to the mature-differentiated HCEC contents: high-maturity group: > 70%, middle-maturity group: 10% to 70%, low-maturity group: < 10%. The successful rate of ECD maintained at 1500 cells/mm2 at 36 months postoperative was analyzed using the log-rank test.
UNASSIGNED: Endothelial cell density and ECL at 36 months postoperative.
UNASSIGNED: The 68 included patients (mean [standard deviation] age 68.1 [13.6] years, 47.1% women, 52.9% DSAEK). The high, middle, and low-maturity groups included 17, 32, and 19 eyes, respectively. At 36 months postoperative, the mean (standard deviation) ECD significantly decreased to 911 (388) cells/mm2 by 66% in the low-maturity group, compared with 1604 (436) by 40% and 1424 (613) cells/mm2 by 50% in the high and middle-maturity groups (P < 0.001 and P = 0.007, respectively) and the low-maturity group significantly failed to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001). Additional ECD analysis for patients who underwent DSAEK alone displayed a significant failure to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001).
UNASSIGNED: The high content of mature-differentiated HCECs expressed in culture by the donor peripheral cornea was coincident with low ECL, suggesting that a high-maturity CEC content predicts long-term graft survival. Understanding the molecular mechanism for maintaining HCEC maturity could elucidate the mechanism of ECL after corneal transplantation and aid in developing effective interventions.
UNASSIGNED: Proprietary or commercial disclosure may be found after the references.
UNASSIGNED: Prospective cohort study.
UNASSIGNED: This cohort study was conducted at Baptist Eye Institute, Kyoto, Japan, between October 2014 and October 2016. It included 68 patients with a 36-month follow-up period who had undergone successful Descemet stripping automated endothelial keratoplasty (DSAEK) or penetrating keratoplasty.
UNASSIGNED: Human CECs (HCECs) from remaining peripheral donor corneas were cultured and evaluated for maturity by surface markers (CD166+, CD44-/dull, CD24-, and CD105-) using fluorescence-activated cell sorting. Postoperative ECD was assessed according to the mature-differentiated HCEC contents: high-maturity group: > 70%, middle-maturity group: 10% to 70%, low-maturity group: < 10%. The successful rate of ECD maintained at 1500 cells/mm2 at 36 months postoperative was analyzed using the log-rank test.
UNASSIGNED: Endothelial cell density and ECL at 36 months postoperative.
UNASSIGNED: The 68 included patients (mean [standard deviation] age 68.1 [13.6] years, 47.1% women, 52.9% DSAEK). The high, middle, and low-maturity groups included 17, 32, and 19 eyes, respectively. At 36 months postoperative, the mean (standard deviation) ECD significantly decreased to 911 (388) cells/mm2 by 66% in the low-maturity group, compared with 1604 (436) by 40% and 1424 (613) cells/mm2 by 50% in the high and middle-maturity groups (P < 0.001 and P = 0.007, respectively) and the low-maturity group significantly failed to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001). Additional ECD analysis for patients who underwent DSAEK alone displayed a significant failure to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001).
UNASSIGNED: The high content of mature-differentiated HCECs expressed in culture by the donor peripheral cornea was coincident with low ECL, suggesting that a high-maturity CEC content predicts long-term graft survival. Understanding the molecular mechanism for maintaining HCEC maturity could elucidate the mechanism of ECL after corneal transplantation and aid in developing effective interventions.
UNASSIGNED: Proprietary or commercial disclosure may be found after the references.
摘要：
未经证实：角膜内皮细胞密度（ECD）在角膜移植后逐渐降低，生物物理，或免疫机制。我们的目的是评估培养中供体角膜内皮细胞（CEC）成熟度与成功角膜移植后术后内皮细胞丢失（ECL）之间的关系。
未经评估：前瞻性队列研究。
未经评估：这项队列研究在浸信会眼科研究所进行，京都,Japan,2014年10月至2016年10月。它包括68例患者，随访期36个月，他们成功进行了Descemet剥离自动内皮角膜移植术（DSAEK）或穿透性角膜移植术。
UNASSIGNED：培养来自剩余外周供体角膜的人类CECs（HCECs），并通过表面标记（CD166，CD44-/暗淡，CD24-,和CD105-）使用荧光激活的细胞分选。术后ECD根据成熟分化HCEC含量进行评估：高成熟组：>70%，中等成熟度组：10％至70％，低成熟度组：<10%。使用对数秩检验分析术后36个月ECD维持在1500个细胞/mm2的成功率。
未授权：术后36个月时的内皮细胞密度和ECL。
未经评估：68名患者（平均[标准差]年龄68.1[13.6]岁，47.1％的妇女，52.9%DSAEK）。高，中间,低成熟度组包括17、32和19只眼，分别。术后36个月，在低成熟度组中，平均（标准偏差）ECD显着降低至911（388）细胞/mm2，降低了66％，与1604（436）的40％和1424（613）细胞/mm2的50％相比，高和中成熟组（分别为P<0.001和P=0.007）和低成熟组明显未能在术后36个月维持ECD在1500个细胞/mm2（P<0.001）。单独接受DSAEK的患者的其他ECD分析显示，在术后36个月时，ECD明显无法维持在1500个细胞/mm2（P<0.001）。
UASSIGNED：供体外周角膜在培养物中表达的成熟分化HCECs含量高，ECL低，表明高成熟度CEC含量可预测移植物的长期存活。了解维持HCEC成熟度的分子机制可以阐明角膜移植后ECL的机制，并有助于开发有效的干预措施。
UNASSIGNED：在参考文献之后可以找到专有或商业披露。
未经评估：前瞻性队列研究。
未经评估：这项队列研究在浸信会眼科研究所进行，
UNASSIGNED：培养来自剩余外周供体角膜的人类CECs（HCECs），并通过表面标记（CD166，
未授权：术后36个月时的内皮细胞密度和ECL。
未经评估：68名患者（平均[标准差]年龄68.1[13.6]岁，
UASSIGNED：供体外周角膜在培养物中表达的成熟分化HCECs含量高，ECL低，
UNASSIGNED：在参考文献之后可以找到专有或商业披露。
