PDF(Pubmed)
Abstract：
UNASSIGNED: Bovine coronavirus (BCoV) is a causative agent of enteric and respiratory diseases in cattle. Despite its importance for animal health, no data is available on its prevalence in Poland. The aim of the study was to determine the virus\' seroprevalence, identify risk factors of BCoV exposure in selected cattle farms and investigate the genetic variability of circulating strains.
UNASSIGNED: Serum and nasal swab samples were collected from 296 individuals from 51 cattle herds. Serum samples were tested with ELISA for the presence of BCoV-, bovine herpesvirus-1 (BoHV-1)- and bovine viral diarrhoea virus (BVDV)-specific antibodies. The presence of those viruses in nasal swabs was tested by real-time PCR assays. Phylogenetic analysis was performed using fragments of the BCoV S gene.
UNASSIGNED: Antibodies specific to BCoV were found in 215 (72.6%) animals. Seropositivity for BCoV was more frequent (P>0.05) in calves under 6 months of age, animals with respiratory signs coinfected with BoHV-1 and BVDV and increased with herd size. In the final model, age and herd size were established as risk factors for BCoV-seropositivity. Genetic material of BCoV was found in 31 (10.5%) animals. The probability of BCoV detection was the highest in medium-sized herds. Polish BCoVs showed high genetic homology (98.3-100%) and close relatedness to European strains.
UNASSIGNED: Infections with BCoV were more common than infections with BoHV-1 and BVDV. Bovine coronavirus exposure and shedding show age- and herd density-dependence.
UNASSIGNED: Serum and nasal swab samples were collected from 296 individuals from 51 cattle herds. Serum samples were tested with ELISA for the presence of BCoV-, bovine herpesvirus-1 (BoHV-1)- and bovine viral diarrhoea virus (BVDV)-specific antibodies. The presence of those viruses in nasal swabs was tested by real-time PCR assays. Phylogenetic analysis was performed using fragments of the BCoV S gene.
UNASSIGNED: Antibodies specific to BCoV were found in 215 (72.6%) animals. Seropositivity for BCoV was more frequent (P>0.05) in calves under 6 months of age, animals with respiratory signs coinfected with BoHV-1 and BVDV and increased with herd size. In the final model, age and herd size were established as risk factors for BCoV-seropositivity. Genetic material of BCoV was found in 31 (10.5%) animals. The probability of BCoV detection was the highest in medium-sized herds. Polish BCoVs showed high genetic homology (98.3-100%) and close relatedness to European strains.
UNASSIGNED: Infections with BCoV were more common than infections with BoHV-1 and BVDV. Bovine coronavirus exposure and shedding show age- and herd density-dependence.
摘要：
未经批准：牛冠状病毒（BCoV）是牛肠道和呼吸道疾病的病原体。尽管它对动物健康很重要，在波兰没有关于其患病率的数据.该研究的目的是确定病毒的血清阳性率，在选定的养牛场中确定BCoV暴露的危险因素，并研究循环菌株的遗传变异性。
未经证实:从51个牛群的296个个体收集血清和鼻拭子样品。血清样品用ELISA检测是否存在BCoV-，牛疱疹病毒-1(BoHV-1)-和牛病毒性腹泻病毒(BVDV)-特异性抗体。通过实时PCR测定法测试了这些病毒在鼻拭子中的存在。使用BCoVS基因的片段进行系统发育分析。
未经证实:在215只(72.6%)动物中发现了BCoV特异性抗体。在6月龄以下的小牛中，BCoV的血清阳性更常见（P>0.05）。具有呼吸体征的动物同时感染了BoHV-1和BVDV，并随着牛群的大小而增加。在最终模型中，年龄和群体规模被确定为BCoV血清阳性的危险因素。在31只（10.5％）动物中发现了BCoV的遗传物质。在中型牛群中，BCoV检测的可能性最高。波兰BCoV表现出很高的遗传同源性（98.3-100％），并且与欧洲菌株密切相关。
未经证实：BCoV感染比BoHV-1和BVDV感染更常见。牛冠状病毒的暴露和脱落表现出年龄和群体密度依赖性。
未经证实:从51个牛群的296个个体收集血清和鼻拭子样品。
未经证实:在215只(72.6%)动物中发现了BCoV特异性抗体。
未经证实：BCoV感染比BoHV-1和BVDV感染更常见。
